ArticleScientific reports2017
Efficient and rapid generation of large genomic variants in rats and mice using CRISMERE.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Ripply3 overdosage induces mid-face shortening through Tbx1 downregulation in Down syndrome models.PLoS genetics · 2025Article
- CRISPR/Cas technologies in pancreatic cancer research and therapeutics: recent advances and future outlook.Discover oncology · 2025Review
- Genome engineering with Cas9 and AAV repair templates, successes and pitfalls.Mammalian genome : official journal of the International Mammalian Genome Society · 2025Review
- Long-read sequencing for fast and robust identification of correct genome-edited alleles: PCR-based and Cas9 capture methods.PLoS genetics · 2024Article
- Genotyping Protocols for Genetically Engineered Mice.Current protocols · 2023Article
- Production of large, defined genome modifications in rats by targeting rat embryonic stem cells.Stem cell reports · 2023Article
- CRISMERE Chromosome Engineering in Mouse and Rat.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Gene Editing in Mouse Zygotes Using the CRISPR/Cas9 System.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Ts66Yah, a mouse model of Down syndrome with improved construct and face validity.Disease models & mechanisms · 2022Article
- Cas9-induced large deletions and small indels are controlled in a convergent fashion.Nature communications · 2022Article
- Importing genetically altered animals: ensuring quality.Mammalian genome : official journal of the International Mammalian Genome Society · 2022Review
- Progress towards completing the mutant mouse null resource.Mammalian genome : official journal of the International Mammalian Genome Society · 2022Article
- Recent Advances in the Production of Genome-Edited Rats.International journal of molecular sciences · 2022Review
- A transchromosomic rat model with human chromosome 21 shows robust Down syndrome features.American journal of human genetics · 2022Article
- Screening and validation of genome-edited animals.Laboratory animals · 2022Article
- DAJIN enables multiplex genotyping to simultaneously validate intended and unintended target genome editing outcomes.PLoS biology · 2022Article
- Advances in mouse genetics for the study of human disease.Human molecular genetics · 2021Review
- High-throughput genotyping of high-homology mutant mouse strains by next-generation sequencing.Methods (San Diego, Calif.) · 2021Article
- All Creatures Great and Small: New Approaches for Understanding Down Syndrome Genetics.Trends in genetics : TIG · 2021Review
- Basic and Preclinical Research for Personalized Medicine.Journal of personalized medicine · 2021Review
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Modelling Down syndrome (DS) in mouse has been crucial for the understanding of the disease and the evaluation of therapeutic targets. Nevertheless, the modelling so far has been limited to the mouse and, even in this model, generating duplication of genomic regions has been labour intensive and time consuming. We developed the CRISpr MEdiated REarrangement (CRISMERE) strategy, which takes advantage of the CRISPR/Cas9 system, to generate most of the desired rearrangements from a single experiment at much lower expenses and in less than 9 months. Deletions, duplications, and inversions of genomic regions as large as 24.4 Mb in rat and mouse founders were observed and germ line transmission was confirmed for fragment as large as 3.6 Mb. Interestingly we have been able to recover duplicated regions from founders in which we only detected deletions. CRISMERE is even more powerful than anticipated it allows the scientific community to manipulate the rodent and probably other genomes in a fast and efficient manner which was not possible before.
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