ArticleThe Journal of experimental medicine1988
The effect of phenolic glycolipid-1 from Mycobacterium leprae on the antimicrobial activity of human macrophages.
Article in The Journal of experimental medicine, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 90 citations in OpenAlex.
- Trial
- Cell Biology of Intracellular Adaptation ofMicrobiology spectrum · 2019Review
- FAD-sequestering proteins protect mycobacteria against hypoxic and oxidative stress.The Journal of biological chemistry · 2019Article
- Mycobacterium tuberculosis arrests host cycle at the G1/S transition to establish long term infection.PLoS pathogens · 2017Article
- MicroRNA-21 targets the vitamin D-dependent antimicrobial pathway in leprosy.Nature medicine · 2012Article
- Modulation of the cytokine response in human monocytes by mycobacterium leprae phenolic glycolipid-1.Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research · 2012Article
- Mycobacterium leprae phenolglycolipid-1 expressed by engineered M. bovis BCG modulates early interaction with human phagocytes.PLoS pathogens · 2010Article
- Deciphering the genetic bases of the structural diversity of phenolic glycolipids in strains of the Mycobacterium tuberculosis complex.The Journal of biological chemistry · 2008Article
- Intact pks15/1 in non-W-Beijing Mycobacterium tuberculosis isolates.Emerging infectious diseases · 2006Article
- MmpL8 is required for sulfolipid-1 biosynthesis and Mycobacterium tuberculosis virulence.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Structural and functional characterization of the Pseudomonas hydroperoxide resistance protein Ohr.The EMBO journal · 2002Article
- Innate immunity to Mycobacterium tuberculosis.Clinical microbiology reviews · 2002Review
- Regulation of expression of major histocompatibility antigens by bovine macrophages infected with Mycobacterium avium subsp. paratuberculosis or Mycobacterium avium subsp. avium.Infection and immunity · 2001Article
- Preliminary characterization of a Mycobacterium abscessus mutant in human and murine models of infection.Infection and immunity · 1999Article
- A novel antioxidant gene from Mycobacterium tuberculosis.The Journal of experimental medicine · 1997Article
- Increased intracellular survival of Mycobacterium smegmatis containing the Mycobacterium leprae thioredoxin-thioredoxin reductase gene.Infection and immunity · 1997Article
- Role of oxidants in microbial pathophysiology.Clinical microbiology reviews · 1997Review
- Identification of the surface-exposed lipids on the cell envelopes of Mycobacterium tuberculosis and other mycobacterial species.Journal of bacteriology · 1996Article
- Antigen-coated latex particles as a model system for probing monocyte responses in leprosy.Infection and immunity · 1993Article
- Production of monoclonal antibody to a phenolic glycolipid of Mycobacterium tuberculosis and its use in detection of the antigen in clinical isolates.Journal of clinical microbiology · 1992Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Purified PGL-1 and dPGL from M. leprae can prevent bacterial killing by intact phagocytes and cell-free antimicrobial systems. Both glycolipids completely abolished the antimicrobial effect of the acetaldehyde-XO-Fe2+ system. Because the cytotoxicity of this system is inhibited by catalase, SOD, mannitol, and ethanol, but not by heated SOD or catalase, these data suggest that toxicity is due to OH. generated by the Haber-Weiss reaction. That the antimicrobial killing in the XO system is completely blocked by the addition of PGL-1 or dPGL suggests that these glycolipids can act as OH. scavengers. A modest protective effect against the cytotoxicity of the MPO-H2O2-halide system by both PGL-1 and dPGL was also observed. The antimicrobial activity of the MPO system was abolished with chloride, but not iodide, as the halide. The effect of the M. leprae-derived glycolipid on bacterial killing by intact phagocytes was examined. Two linking antibodies were used to bind the dPGL to a rapidly growing test organism, S. aureus, a murine IgM mAb specific for the terminal glycoside of PGL-1, and a rabbit IgG anti-mouse IgM which bound the staphylococcal protein A via its Fc region. Examination by transmission EM of human monocyte-derived macrophages which had ingested staphylococci either coated with both antibodies and dPGL, or coated only with the IgG and IgM antibodies, demonstrated the presence of bacteria in phagosomes of control and IFN-gamma-activated macrophages. Activation of the macrophage monolayers by pretreatment with IFN-gamma markedly increased their staphylocidal activity. When dPGL coated staphylococci were ingested, killing by both control and IFN-gamma-activated macrophages was completely blocked. These results, suggesting that PGL-1 can scavenge reactive oxygen species and prevent microbial death within the phagosome, may in part explain the intracellular survival of M. leprae in certain cell types.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.