ArticlePeerJ2017
An extremely sensitive nested PCR-RFLP mitochondrial marker for detection and identification of salmonids in eDNA from water samples.
Article in PeerJ, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 32 citations in OpenAlex.
- Detection the eDNA ofAnimals : an open access journal from MDPI · 2022Article
- Benefits and limitations of a new genome-based PCR-RFLP genotyping assay (GB-RFLP): A SNP-based detection method for identification of species in extremely young adaptive radiations.Ecology and evolution · 2022Article
- Reinforcement of Environmental DNA Based Methods (Biology · 2021Review
- An application of PCR-RFLP species identification assay for environmental DNA detection.PeerJ · 2019Article
- Environmental DNA for freshwater fish monitoring: insights for conservation within a protected area.PeerJ · 2018Article
- GoFish: A versatile nested PCR strategy for environmental DNA assays for marine vertebrates.PloS one · 2018Article
- Evaluating freshwater macroinvertebrates from eDNA metabarcoding: A river Nalón case study.PloS one · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSalmonids are native from the North Hemisphere but have been introduced for aquaculture and sport fishing in the South Hemisphere and inhabit most rivers and lakes in temperate and cold regions worldwide. Five species are included in the Global Invasive Species Database: rainbow trout
methodsHere we have developed a method to identify salmonid species directly from water samples, focusing on the Iberian Peninsula as a case study. We have designed nested Salmonidae-specific primers within the 16S rDNA region. From these primers and a PCR-RFLP procedure the target species can be unequivocally identified from DNA extracted from water samples.
resultsThe method was validated in aquarium experiments and in the field with water from watersheds with known salmonid populations. Finally, the method was applied to obtain a global view of the Salmonidae community in Nalón River (north coast of Spain). DISCUSSION: This new powerful, very sensitive (identifying the species down to 10 pg DNA/ml water) and economical tool can be applied for monitoring the presence of salmonids in a variety of situations, from checking upstream colonization after removal of river barriers to monitoring potential escapes from fish farms.
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Registered trials
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