Evidence map›Paper›PMID 28265068›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2017

Cas9-mediated genome editing in the methanogenic archaeon

Dipti D Nayak, William W Metcalf

Abstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.

0numbers the graph read from it
0cells of the map it votes in
82citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

82 citing papers in PubMed.

  1. Article
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  6. Review
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  8. Article
  9. Methanogenic archaea encoding Pyrrolysine maintain ambiguous amber codon usage.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
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  18. Review
  19. All-in-One COAccounts of chemical research · 2024
    Review
  20. Article

22 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

2 authors.

Dipti D NayakCarl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801.ORCID 0000-0002-8390-7251
William W MetcalfDepartment of Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL 61801 metcalf@illinois.edu.ORCID 0000-0002-0182-0671

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Although Cas9-mediated genome editing has proven to be a powerful genetic tool in eukaryotes, its application in Bacteria has been limited because of inefficient targeting or repair; and its application to Archaea has yet to be reported. Here we describe the development of a Cas9-mediated genome-editing tool that allows facile genetic manipulation of the slow-growing methanogenic archaeon

Indexed as

CRISPR-Cas SystemsGene EditingGenomeDNA RepairGene Expression Regulation, ArchaealGenes, EssentialGenetic VectorsGenomicsMethanosarcinaMutationPlasmidsRNA, Guide, CRISPR-Cas SystemsRNA, Guide, CRISPR-Cas SystemsArchaeaCas9geneticsmethanogensMethanosarcina

Identifiers

PMID28265068
PMCPMC5358397

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.