ArticlePloS one2017
Phylogenetic diversity and in situ detection of eukaryotes in anaerobic sludge digesters.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Substrate-driven phage community structure and phage-host dynamics in anaerobic digesters.Environmental microbiome · 2026Article
- Characterization of Dominant Heterotrophic Flagellates in Anaerobic Digesters Using Combined Culture-based and Metabarcoding Approaches.Microbial ecology · 2026Article
- Metabolic Potential of Candidatus Saccharimonadia Including Rare Lineages in Activated Sludge.Environmental microbiology reports · 2025Article
- Developments in Microbial Communities and Interaction Networks in Sludge Treatment Ecosystems During the Transition from Anaerobic to Aerobic Conditions.Microorganisms · 2025Article
- From lab to life: technological innovations in transforming cancer metastasis detection and therapy.Discover oncology · 2025Review
- Occurrence and temporal dynamics of denitrifying protist endosymbionts in the wastewater microbiome.ISME communications · 2025Article
- Eukaryotic community composition and dynamics during solid waste decomposition.Applied microbiology and biotechnology · 2022Article
- Diversity of Candidatus Patescibacteria in Activated Sludge Revealed by a Size-Fractionation Approach.Microbes and environments · 2022Article
- Optimizing the hybridization chain reaction-fluorescence in situ hybridization (HCR-FISH) protocol for detection of microbes in sediments.Marine life science & technology · 2021Article
- Colonization kinetics and implantation follow-up of the sewage microbiome in an urban wastewater treatment plant.Scientific reports · 2020Article
- Food selectivity of anaerobic protists and direct evidence for methane production using carbon from prey bacteria by endosymbiotic methanogen.The ISME journal · 2020Article
- Uncovering Viable Microbiome in Anaerobic Sludge Digesters by Propidium Monoazide (PMA)-PCR.Microbial ecology · 2020Article
- Temporal variation of eukaryotic community structures in UASB reactor treating domestic sewage as revealed by 18S rRNA gene sequencing.Scientific reports · 2019Article
- Ten reasons why a sequence-based nomenclature is not useful for fungi anytime soon.IMA fungus · 2018Article
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5 authors.
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Abstract
Eukaryotic communities in aerobic wastewater treatment processes are well characterized, but little is known about them in anaerobic processes. In this study, abundance, diversity and morphology of eukaryotes in anaerobic sludge digesters were investigated by quantitative real-time PCR (qPCR), 18S rRNA gene clone library construction and catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH). Samples were taken from four different anaerobic sludge digesters in Japan. Results of qPCR of rRNA genes revealed that Eukarya accounted from 0.1% to 1.4% of the total number of microbial rRNA gene copy numbers. The phylogenetic affiliations of a total of 251 clones were Fungi, Alveolata, Viridiplantae, Amoebozoa, Rhizaria, Stramenopiles and Metazoa. Eighty-five percent of the clones showed less than 97.0% sequence identity to described eukaryotes, indicating most of the eukaryotes in anaerobic sludge digesters are largely unknown. Clones belonging to the uncultured lineage LKM11 in Cryptomycota of Fungi were most abundant in anaerobic sludge, which accounted for 50% of the total clones. The most dominant OTU in each library belonged to either the LKM11 lineage or the uncultured lineage A31 in Alveolata. Principal coordinate analysis indicated that the eukaryotic and prokaryotic community structures were related. The detection of anaerobic eukaryotes, including the members of the LKM11 and A31 lineages in anaerobic sludge digesters, by CARD-FISH revealed their sizes in the range of 2-8 μm. The diverse and uncultured eukaryotes in the LKM11 and the A31 lineages are common and ecologically relevant members in anaerobic sludge digester.
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