ArticlePLoS genetics2017
Overexpression of the WOX gene STENOFOLIA improves biomass yield and sugar release in transgenic grasses and display altered cytokinin homeostasis.
Article in PLoS genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 54 citations in OpenAlex.
- Overview of molecular mechanisms of plant leaf development: a systematic review.Frontiers in plant science · 2023Pooled it
- Guardians of Water and Gas Exchange: Adaptive Dynamics of Stomatal Development and Patterning.Plants (Basel, Switzerland) · 2025Review
- Improving crop yield potential: Underlying biological processes and future prospects.Food and energy security · 2023Review
- WOX family transcriptional regulators modulate cytokinin homeostasis during leaf blade development in Medicago truncatula and Nicotiana sylvestris.The Plant cell · 2022Article
- Leaf Development inGenes · 2022Review
- Auxin/Cytokinin Antagonistic Control of the Shoot/Root Growth Ratio and Its Relevance for Adaptation to Drought and Nutrient Deficiency Stresses.International journal of molecular sciences · 2022Review
- Overexpression of PvWOX3a in switchgrass promotes stem development and increases plant height.Horticulture research · 2021Article
- Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs.International journal of molecular sciences · 2021Review
- The WOX family transcriptional regulator SlLAM1 controls compound leaf and floral organ development in Solanum lycopersicum.Journal of experimental botany · 2021Article
- Differential expression of SlKLUH controlling fruit and seed weight is associated with changes in lipid metabolism and photosynthesis-related genes.Journal of experimental botany · 2021Article
- Hydrogen sulfide promotes flowering in heading Chinese cabbage by S-sulfhydration of BraFLCs.Horticulture research · 2021Article
- The GA 20-Oxidase Encoding GeneFrontiers in plant science · 2021Article
- Influence of SwitchgrassFrontiers in plant science · 2021Article
- SMALL LEAF AND BUSHY1 controls organ size and lateral branching by modulating the stability of BIG SEEDS1 in Medicago truncatula.The New phytologist · 2020Article
- Genome-Wide Identification of the Physic Nut WUSCHEL-Related Homeobox Gene Family and Functional Analysis of the Abiotic Stress Responsive GeneFrontiers in genetics · 2020Article
- The MYB Activator WHITE PETAL1 Associates with MtTT8 and MtWD40-1 to Regulate Carotenoid-Derived Flower Pigmentation inThe Plant cell · 2019Article
- HEADLESS, a WUSCHEL homolog, uncovers novel aspects of shoot meristem regulation and leaf blade development in Medicago truncatula.Journal of experimental botany · 2019Article
- Modulation of Asymmetric Division Diversity through Cytokinin and SPEECHLESS Regulatory Interactions in the Arabidopsis Stomatal Lineage.Developmental cell · 2018Article
- A Core Regulatory Pathway Controlling Rice Tiller Angle Mediated by theThe Plant cell · 2018Article
- The functional balance between thePlant biotechnology (Tokyo, Japan) · 2018Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lignocellulosic biomass can be a significant source of renewable clean energy with continued improvement in biomass yield and bioconversion strategies. In higher plants, the leaf blade is the central energy convertor where solar energy and CO2 are assimilated to make the building blocks for biomass production. Here we report that introducing the leaf blade development regulator STENOFOLIA (STF), a WOX family transcription factor, into the biofuel crop switchgrass, significantly improves both biomass yield and sugar release. We found that STF overexpressing switchgrass plants produced approximately 2-fold more dry biomass and release approximately 1.8-fold more solubilized sugars without pretreatment compared to controls. The biomass increase was attributed mainly to increased leaf width and stem thickness, which was also consistent in STF transgenic rice and Brachypodium, and appeared to be caused by enhanced cell proliferation. STF directly binds to multiple regions in the promoters of some cytokinin oxidase/dehydrogenase (CKX) genes and represses their expression in all three transgenic grasses. This repression was accompanied by a significant increase in active cytokinin content in transgenic rice leaves, suggesting that the increase in biomass productivity and sugar release could at least in part be associated with improved cytokinin levels caused by repression of cytokinin degrading enzymes. Our study provides a new tool for improving biomass feedstock yield in bioenergy crops, and uncovers a novel mechanistic insight in the function of STF, which may also apply to other repressive WOX genes that are master regulators of several key plant developmental programs.
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