ArticleJournal of molecular biology2017
Osmotic Shock Induced Protein Destabilization in Living Cells and Its Reversal by Glycine Betaine.
Article in Journal of molecular biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Protein aggregation as a bistable switch in bacterial cell fate: from adaptive dormancy to cytotoxic death.Frontiers in microbiology · 2026Review
- Glycopolymers stabilize protein folding and protein-protein interactions via enthalpic interactions.Protein science : a publication of the Protein Society · 2026Article
- Article
- Crowding-induced stabilization and destabilization in a single protein.Protein science : a publication of the Protein Society · 2025Article
- Amino acids modulate liquid-liquid phase separation in vitro and in vivo by regulating protein-protein interactions.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Optimized partial freezing protocol enables 10-day storage of rat livers.Scientific reports · 2024Article
- Expanding the tool box for native structural biology:Science advances · 2024Article
- Sub-zero non-freezing of vascularized composite allografts in a rodent partial hindlimb model.Cryobiology · 2024Article
- Structure and mechanism of the osmoregulated choline transporter BetT.Science advances · 2024Article
- Trimethylamine N-oxide (TMAO) doubly locks the hydrophobic core and surfaces of protein against desiccation stress.Protein science : a publication of the Protein Society · 2024Article
- The substrate-binding domains of the osmoregulatory ABC importer OpuA transiently interact.eLife · 2024Article
- Detrimental Impacts of Pharmaceutical Excipient PEG400 on Gut Microbiota and Metabolome in Healthy Mice.Molecules (Basel, Switzerland) · 2023Article
- Chemical interactions modulate λProtein science : a publication of the Protein Society · 2023Article
- Physiological and regulatory convergence between osmotic and nutrient stress responses in microbes.Current opinion in cell biology · 2023Review
- Water Stress-Driven Changes in Bacterial Cell Surface Properties.Applied and environmental microbiology · 2022Article
- Protein Fibrillation under Crowded Conditions.Biomolecules · 2022Review
- Radio Signals from Live Cells: The Coming of Age of In-Cell Solution NMR.Chemical reviews · 2022Review
- L-Proline Synthesis Mutants ofFrontiers in microbiology · 2022Article
- Melatonin: Regulation of Biomolecular Condensates in Neurodegenerative Disorders.Antioxidants (Basel, Switzerland) · 2021Review
- Molecular crowding in single eukaryotic cells: Using cell environment biosensing and single-molecule optical microscopy to probe dependence on extracellular ionic strength, local glucose conditions, and sensor copy number.Methods (San Diego, Calif.) · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Many organisms can adapt to changes in the solute content of their surroundings (i.e., the osmolarity). Hyperosmotic shock causes water efflux and a concomitant reduction in cell volume, which is countered by the accumulation of osmolytes. This volume reduction increases the crowded nature of the cytoplasm, which is expected to affect protein stability. In contrast to traditional theory, which predicts that more crowded conditions can only increase protein stability, recent work shows that crowding can destabilize proteins through transient attractive interactions. Here, we quantify protein stability in living Escherichia coli cells before and after hyperosmotic shock in the presence and absence of the osmolyte, glycine betaine. The 7-kDa N-terminal src-homology 3 domain of Drosophila signal transduction protein drk is used as the test protein. We find that hyperosmotic shock decreases SH3 stability in cells, consistent with the idea that transient attractive interactions are important under physiologically relevant crowded conditions. The subsequent uptake of glycine betaine returns SH3 to the stability observed without osmotic shock. These results highlight the effect of transient attractive interactions on protein stability in cells and provide a new explanation for why stressed cells accumulate osmolytes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.