ArticleScientific reports2017
Identification of stable housekeeping genes in response to ionizing radiation in cancer research.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 36 citations in OpenAlex.
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- YWHAZ and TBP are potential reference gene candidates for qPCR analysis of response to radiation therapy in colorectal cancer.Scientific reports · 2023Article
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- Identification of appropriate housekeeping genes for gene expression studies in human renal cell carcinoma under hypoxic conditions.Molecular biology reports · 2022Article
- Increased biting rate and decreased Wolbachia density in irradiated Aedes mosquitoes.Parasites & vectors · 2022Article
- Functional Transient Receptor Potential Ankyrin 1 and Vanilloid 1 Ion Channels Are Overexpressed in Human Oral Squamous Cell Carcinoma.International journal of molecular sciences · 2022Article
- Identification and Validation of Common Reference Genes for Normalization of Esophageal Squamous Cell Carcinoma Gene Expression Profiles.BioMed research international · 2022Article
- RPS13, a potential universal reference gene for normalisation of gene expression in multiple human normal and cancer tissue samples.Molecular biology reports · 2021Article
- Gene Expression Studies in Formalin-Fixed Paraffin-Embedded Samples of Cutaneous Cancer: The Need for Reference Genes.Current issues in molecular biology · 2021Article
- Response of human macrophages to gamma radiation is mediated via expression of endogenous retroviruses.PLoS pathogens · 2021Article
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- Development of an In Situ Cancer Vaccine via Combinational Radiation and Bacterial-Membrane-Coated Nanoparticles.Advanced materials (Deerfield Beach, Fla.) · 2019Article
- Reference Gene Expression in Adipose-Derived Stromal Cells Undergoing Adipogenic Differentiation.Tissue engineering. Part C, Methods · 2019Article
- Circular RNA cTFRC acts as the sponge of MicroRNA-107 to promote bladder carcinoma progression.Molecular cancer · 2019Article
- Karyopherins in cancer.Current opinion in cell biology · 2018Review
- Cancer Hallmarks Analytics Tool (CHAT): a text mining approach to organize and evaluate scientific literature on cancer.Bioinformatics (Oxford, England) · 2017Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Housekeeping genes (HKGs) are essential for basic maintenance of a variety of cellular processes. They ideally maintain uniform expression independent of experimental conditions. However, the effects of ionizing radiation (IR) on HKG expression is unclear. Statistical algorithms, geNorm and Normfinder were used for estimating the stability of HKGs as raw quantification cycle (Cq) values were not a reliable factor for normalization. Head and neck, non-small lung and pancreas cells were exposed to 2, 4 and 6 Gy IR doses and expression of fourteen HKGs was measured at 5 min to 48 h post-irradiation within a given tissue. Paired and single cell line analyses under these experimental conditions identified TATA-Box Binding Protein (TBP) and Importin 8 (IPO8) to be stable in non-small cell lung cancer. In addition to these two genes, Ubiquitin C (UBC) in head and neck cancer and Transferrin receptor (TFRC) and β-Glucuronidase (GUSB) in pancreatic cancer were identified to be stable as well. In summary we present a resource for top ranked five stable HKGs and their transcriptional behavior in commonly used cancer model cell lines and suggest the use of multiple HKGs under radiation treatment conditions is a reliable metric for quantifying gene expression.
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