ArticleScientific reports2017
A biomimetic gelatin-based platform elicits a pro-differentiation effect on podocytes through mechanotransduction.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- Construction, evaluation, and applications of renal barrier-on-a-chip system.Bioactive materials · 2026Review
- New Dimensions in Diabetic Kidney Disease Research: Advanced Organoids and Organs-on-a-Chip.BME frontiers · 2026Review
- Biomimetic fractal topography enhances podocyte maturation in vitro.Nature communications · 2025Article
- Piezo1, F-Actin Remodeling, and Podocyte Survival and Regeneration.Journal of the American Society of Nephrology : JASN · 2025Article
- Mechanosensitive Differentiation of Human iPS Cell-Derived Podocytes.Bioengineering (Basel, Switzerland) · 2024Article
- Role of biophysics and mechanobiology in podocyte physiology.Nature reviews. Nephrology · 2024Review
- Kidney-on-a-Chip: Mechanical Stimulation and Sensor Integration.Sensors (Basel, Switzerland) · 2022Review
- An ex vivo culture model of kidney podocyte injury reveals mechanosensitive, synaptopodin-templating, sarcomere-like structures.Science advances · 2022Article
- Modeling the Glomerular Filtration Barrier and Intercellular Crosstalk.Frontiers in physiology · 2021Review
- Cell derived extracellular matrix-rich biomimetic substrate supports podocyte proliferation, differentiation and maintenance of native phenotype.Advanced functional materials · 2020Article
- LIM-Nebulette Reinforces Podocyte Structural Integrity by Linking Actin and Vimentin Filaments.Journal of the American Society of Nephrology : JASN · 2020Article
- Enzymatically crosslinked gelatin-laminin hydrogels for applications in neuromuscular tissue engineering.Biomaterials science · 2020Article
- Article
- DLITE Uses Cell-Cell Interface Movement to Better Infer Cell-Cell Tensions.Biophysical journal · 2019Article
- Biomaterials to Mimic and Heal Connective Tissues.Advanced materials (Deerfield Beach, Fla.) · 2019Review
- Engineering renal epithelial cells: programming and directed differentiation towards glomerular podocyte's progenitor and mature podocyte.American journal of translational research · 2019Review
- A Multi-layered Quantitative In Vivo Expression Atlas of the Podocyte Unravels Kidney Disease Candidate Genes.Cell reports · 2018Article
- Similar Biophysical Abnormalities in Glomeruli and Podocytes from Two Distinct Models.Journal of the American Society of Nephrology : JASN · 2018Article
- Cell shape information is transduced through tension-independent mechanisms.Nature communications · 2017Article
- Mechanical forces direct stem cell behaviour in development and regeneration.Nature reviews. Molecular cell biology · 2017Review
Corrections and comments
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Authors and funding
14 authors at 2 institutions in 1 country.
Funding
Abstract
Using a gelatin microbial transglutaminase (gelatin-mTG) cell culture platform tuned to exhibit stiffness spanning that of healthy and diseased glomeruli, we demonstrate that kidney podocytes show marked stiffness sensitivity. Podocyte-specific markers that are critical in the formation of the renal filtration barrier are found to be regulated in association with stiffness-mediated cellular behaviors. While podocytes typically de-differentiate in culture and show diminished physiological function in nephropathies characterized by altered tissue stiffness, we show that gelatin-mTG substrates with Young's modulus near that of healthy glomeruli elicit a pro-differentiation and maturation response in podocytes better than substrates either softer or stiffer. The pro-differentiation phenotype is characterized by upregulation of gene and protein expression associated with podocyte function, which is observed for podocytes cultured on gelatin-mTG gels of physiological stiffness independent of extracellular matrix coating type and density. Signaling pathways involved in stiffness-mediated podocyte behaviors are identified, revealing the interdependence of podocyte mechanotransduction and maintenance of their physiological function. This study also highlights the utility of the gelatin-mTG platform as an in vitro system with tunable stiffness over a range relevant for recapitulating mechanical properties of soft tissues, suggesting its potential impact on a wide range of research in cellular biophysics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.