ArticleCrystal growth & design2016
A Versatile System for High-Throughput In Situ X-ray Screening and Data Collection of Soluble and Membrane-Protein Crystals.
Article in Crystal growth & design, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The trial behind it
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Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- Efficient in situ screening of and data collection from microcrystals in crystallization plates.Acta crystallographica. Section D, Structural biology · 2024Article
- Crystallographic insights into lipid-membrane protein interactions in microbial rhodopsins.Frontiers in molecular biosciences · 2024Article
- Microfluidic rotating-target device capable of three-degrees-of-freedom motion for efficient in situ serial synchrotron crystallography.Journal of synchrotron radiation · 2023Article
- Novel combined crystallization plate for high-throughput crystal screening and in situ data collection at a crystallography beamline.Acta crystallographica. Section F, Structural biology communications · 2021Article
- Low-doseIUCrJ · 2020Article
- 3D-printed holders forJournal of applied crystallography · 2020Article
- Article
- VMXi: a fully automated, fully remote, high-flux in situ macromolecular crystallography beamline.Journal of synchrotron radiation · 2019Article
- High-throughput in situ X-ray screening of and data collection from protein crystals at room temperature and under cryogenic conditions.Nature protocols · 2018Article
- In situ serial crystallography for rapid de novo membrane protein structure determination.Communications biology · 2018Article
- X-ray transparent microfluidic chips for high-throughput screening and optimization ofBiomicrofluidics · 2017Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In recent years, in situ data collection has been a major focus of progress in protein crystallography. Here, we introduce the Mylar in situ method using Mylar-based sandwich plates that are inexpensive, easy to make and handle, and show significantly less background scattering than other setups. A variety of cognate holders for patches of Mylar in situ sandwich films corresponding to one or more wells makes the method robust and versatile, allows for storage and shipping of entire wells, and enables automated crystal imaging, screening, and goniometer-based X-ray diffraction data-collection at room temperature and under cryogenic conditions for soluble and membrane-protein crystals grown in or transferred to these plates. We validated the Mylar in situ method using crystals of the water-soluble proteins hen egg-white lysozyme and sperm whale myoglobin as well as the 7-transmembrane protein bacteriorhodopsin from
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.