ArticleActa pharmacologica Sinica2017
Autophagy inhibitor 3-methyladenine alleviates overload-exercise-induced cardiac injury in rats.
Article in Acta pharmacologica Sinica, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- XopA: a novel type III secretion system effector inMicrobiology spectrum · 2026Article
- The role and possible mechanism of the ferroptosis-related SLC7A11/GSH/GPX4 pathway in myocardial ischemia-reperfusion injury.BMC cardiovascular disorders · 2024Article
- [3-Methyladenine alleviates early renal injury in diabetic mice by inhibiting AKT signaling].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2024Article
- Review
- Does Exercise Regulate Autophagy in Humans? A Systematic Review and Meta-Analysis.Autophagy reports · 2023Review
- Exercise sustains the hallmarks of health.Journal of sport and health science · 2023Review
- Peroxisome Proliferator-Activated ReceptorPPAR research · 2023Article
- Regulation of the autophagy plays an important role in acute kidney injury induced acute lung injury.Renal failure · 2022Article
- Role of adiponectin in osteoarthritis.Frontiers in cell and developmental biology · 2022Review
- Novel insights into exhaustive exercise-induced myocardial injury: Focusing on mitochondrial quality control.Frontiers in cardiovascular medicine · 2022Review
- Autophagy and Endoplasmic Reticulum Stress during Onset and Progression of Arrhythmogenic Cardiomyopathy.Cells · 2021Article
- Ivabradine protects rats against myocardial infarction through reinforcing autophagy via inhibiting PI3K/AKT/mTOR/p70S6K pathway.Bioengineered · 2021Article
- Autophagy positively regulates Wnt signaling in mice with diabetic retinopathy.Experimental and therapeutic medicine · 2021Article
- Impact of Different Physical Exercises on the Expression of Autophagy Markers in Mice.International journal of molecular sciences · 2021Article
- Article
- Exercise combined with trimetazidine improves anti-fatal stress capacity through enhancing autophagy and heat shock protein 70 of myocardium in mice.International journal of medical sciences · 2021Article
- Moderate, but Not Excessive, Training Attenuates Autophagy Machinery in Metabolic Tissues.International journal of molecular sciences · 2020Article
- Exercise-mediated regulation of autophagy in the cardiovascular system.Journal of sport and health science · 2020Review
- Prevention of Doxorubicin-Induced Autophagy Attenuates Oxidative Stress and Skeletal Muscle Dysfunction.Antioxidants (Basel, Switzerland) · 2020Article
- Effect of moxibustion preconditioning on autophagy-related proteins in rats with myocardial ischemia reperfusion injury.Annals of translational medicine · 2019Article
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9 authors.
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Abstract
Overload-exercise (OE) causes myocardial injury through inducing autophagy and apoptosis. In this study we examined whether an autophagy inhibitor 3-methyladenine (3-MA) could alleviate OE-induced cardiac injury. Rats were injected with 3-MA (15 mg/kg, iv) or saline before subjected to various intensities of OE, including no swim (control), 2 h swim (mild-intensity exercise, MIE), 2 h swim with 2.5% body weight overload (moderate OE; MOE), 5% overload (intensive OE; IOE) or 2.5% overload until exhausted (exhaustive OE; EOE). After OE, the hearts were harvested for morphological and biochemiacal analysis. The cardiac morphology, autophagosomes and apoptosis were examined with H&E staining, transmission electron microscopy and TUNEL analysis, respectively. Autophagy-related proteins to (LC3-II/I and Beclin-1) and apoptosis-related proteins (Bcl-2/Bax) were assessed using Western blotting. Our results showed that compared with the control, MIE did not change the morphological structures of the heart tissues that exhibited intact myocardial fibers and neatly arranged cardiomyocytes. However, IOE resulted in irregular arrangement of cardiomyocytes and significantly increased width of cardiomyocytes, whereas EOE caused more swollen and even disrupted cardiomyocytes. In parallel with the increased OE intensity (MOE, IOE, EOE), cardiomyocyte autophagy and apoptosis became more and more prominent, evidenced by the increasing number of autophagosomes and expression levels of LC3-II/I and Beclin-1 as well as the increasing apoptotic cells and decreasing Bcl-2/Bax ratio. 3-MA administration significantly attenuated OE-induced morphological changes of cardiomyocytes as well as all the autophagy- and apoptosis-related abnormalities in MOE, IOE and EOE rats. Thus, the autophagy inhibitor 3-MA could alleviate OE-induced heart injury in rats.
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