ArticleCytokine2017
Impact of freeze-thaw cycles on circulating inflammation marker measurements.
Article in Cytokine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- THBS2/CA19-9 Detecting Pancreatic Ductal Adenocarcinoma at Diagnosis Underperforms in Prediagnostic Detection: Implications for Biomarker Advancement.Cancer prevention research (Philadelphia, Pa.) · 2021Trial
- Reliability and suitability of physiological exercise response and recovery markers.Scientific reports · 2020Trial
- Pro- and Anti-Inflammatory Responses and Clinical Outcomes in Critically Ill Patients With Endotheliopathy: A Cohort Study.Acta anaesthesiologica Scandinavica · 2025Article
- The researcher's guide to selecting biomarkers in mental health studies.BioEssays : news and reviews in molecular, cellular and developmental biology · 2024Review
- Research Bronchoscopies in Critically Ill Research Participants: An Official American Thoracic Society Workshop Report.Annals of the American Thoracic Society · 2023Article
- Relevant Risk Factor and Follow-Up of Lung Nodules in Physical Examination with Low-Dose CT Screening.Iranian journal of public health · 2023Article
- Immunopathology of terminal complement activation and complement C5 blockade creating a pro-survival and organ-protective phenotype in trauma.British journal of pharmacology · 2023Article
- Vaginal cytokine profile and microbiota before and after lubricant use compared with condomless vaginal sex: a preliminary observational study.BMC infectious diseases · 2021Observational
- Article
- Effects of Production Method and Repeated Freeze Thaw Cycles on Cytokine Concentrations and Microbial Contamination in Equine Autologous Conditioned Serum.Frontiers in veterinary science · 2021Article
- Effect of Repeated Freeze-Thaw on Serum Biomarkers Associated with Eye Disease.Medical science monitor : international medical journal of experimental and clinical research · 2018Article
- Circulating Levels of Inflammatory Proteins and Survival in Patients with Gallbladder Cancer.Scientific reports · 2018Article
- Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
backgroundCirculating inflammation markers are being increasingly measured in prospective cohorts to investigate cancer etiology. However, it is unclear how the measurements are affected by the freeze-thaw cycles of the specimens prior to marker analysis.
methodsWe compared concentrations of 45 inflammation markers between paired serum vials of 55 participants in the Prostate, Lung, Colorectal, and Ovarian (PLCO) Cancer Screening Trial that have undergone one (T1), two (T2), and three (T3) freeze-thaw cycles at the time of assay. We computed the difference of analyte concentrations across paired vials (T1 vs. T2, T2 vs. T3) and tested whether the difference deviated from zero using the Wilcoxon signed-rank test. We also calculated Spearman rank correlation and weighted kappa statistics for T1 vs. T2 and T2 vs. T3 comparisons to assess agreement in rank ordering of subjects.
resultsMeasurements between paired T1 and T2 samples were largely similar, with the difference not statistically deviating from zero for 36 of the 45 markers. In contrast, tests of the difference between paired T2 and T3 samples were statistically significant for 36 markers. However, the rank ordering of participants by marker concentration remained largely consistent across T2 and T3 samples, with Spearman correlation coefficients >0.8 for 42 markers and weighted kappas >0.7 for 37 markers.
conclusionWe recommend that studies measuring inflammation markers use previously unthawed specimens to the extent possible, or match on the number of prior freeze-thaw cycles in nested case-control studies.
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