Evidence map›Paper›PMID 28260621›Full record

ArticleVirology2017

Differential regulation of hepatitis B virus core protein expression and genome replication by a small upstream open reading frame and naturally occurring mutations in the precore region.

Li Zong, Yanli Qin, Haodi Jia, Lei Ye, Yongxiang Wang, Jiming Zhang, Jack R Wands, Shuping Tong, Jisu Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.7field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
  2. Article
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  4. Review
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Li ZongLiver Research Center, Rhode Island Hospital, Warren Alpert School of Medicine, Brown University, Providence, RI, USA; Key Lab of Medical Molecular Virology, School of Basic Medical Sciences, Fudan University, Shanghai, China.
Yanli QinDepartment of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China.
Haodi JiaKey Lab of Medical Molecular Virology, School of Basic Medical Sciences, Fudan University, Shanghai, China.
Lei YeKey Lab of Medical Molecular Virology, School of Basic Medical Sciences, Fudan University, Shanghai, China.
Yongxiang WangKey Lab of Medical Molecular Virology, School of Basic Medical Sciences, Fudan University, Shanghai, China.
Jiming ZhangDepartment of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China.
Jack R WandsLiver Research Center, Rhode Island Hospital, Warren Alpert School of Medicine, Brown University, Providence, RI, USA.
Shuping TongLiver Research Center, Rhode Island Hospital, Warren Alpert School of Medicine, Brown University, Providence, RI, USA; Key Lab of Medical Molecular Virology, School of Basic Medical Sciences, Fudan University, Shanghai, China.
Jisu LiLiver Research Center, Rhode Island Hospital, Warren Alpert School of Medicine, Brown University, Providence, RI, USA. Electronic address: ji_su_li_md@brown.edu.
Fudan University · CNHuashan Hospital · CNRhode Island Hospital · US

Funding

Hepatitis B virus vaccine escape mutantsR01AI116639 · NIAID · RHODE ISLAND HOSPITAL · PI TONG, SHUPING · 2015 to 2019
$1.8M
Hepatitis B virus transcriptional interference and liver cancer-related mutationsR21AI113394 · NIAID · RHODE ISLAND HOSPITAL · PI TONG, SHUPING · 2015 to 2016
$443k
Hepatitis B virus genotypes B and CR21AI103648 · NIAID · RHODE ISLAND HOSPITAL · PI TONG, SHUPING · 2013 to 2014
$437k
Control of HBV replication at the step of core and P protein translationR21AI107618 · NIAID · RHODE ISLAND HOSPITAL · PI LI, JISU · 2013 to 2014
$423k
NIAID NIH HHS R01 AI116639NIAID NIH HHS R21 AI103648NIAID NIH HHS R21 AI107618NIAID NIH HHS R21 AI113394
6 · The paper itself

Abstract

Hepatitis B virus (HBV) transcribes two subsets of 3.5-kb RNAs: precore RNA for hepatitis B e antigen (HBeAg) expression, and pregenomic RNA for core and P protein translation as well as genome replication. HBeAg expression could be prevented by mutations in the precore region, while an upstream open reading frame (uORF) has been proposed as a negative regulator of core protein translation. We employed replication competent HBV DNA constructs and transient transfection experiments in Huh7 cells to verify the uORF effect and to explore the alternative function of precore RNA. Optimized Kozak sequence for the uORF or extra ATG codons as present in some HBV genotypes reduced core protein expression. G1896A nonsense mutation promoted more efficient core protein expression than mutated precore ATG, while a +1 frameshift mutation was ineffective. In conclusion, various HBeAg-negative precore mutations and mutations affecting uORF differentially regulate core protein expression and genome replication.

Indexed as

Base SequenceCell Line, TumorCodon, NonsenseDNA ReplicationDNA, ViralFrameshift MutationGene Expression Regulation, ViralHepatitis B, ChronicHepatitis B Core AntigensHepatitis B e AntigensHepatitis B virusHumansOpen Reading FramesPromoter Regions, GeneticProtein PrecursorsViral Core ProteinsCodon, NonsenseDNA, ViralHepatitis B Core AntigensHepatitis B e AntigensProtein PrecursorsViral Core ProteinsCore proteinGenome replicationHepatitis B e antigenHepatitis B virusPrecore mutantPrecore RNAPregenomic RNATranslational control

Identifiers

PMID28260621
PMCPMC5391676
OpenAlexW2591732461

What OpenQuestion holds

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LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.