ArticleJournal of medical microbiology2017
Comparison of culture-dependent and culture-independent molecular methods for characterization of vaginal microflora.
Article in Journal of medical microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A Molecular Approach for Detecting Bacteria and Fungi in Healthcare Environment Aerosols: A Systematic Review.International journal of molecular sciences · 2024Pooled it
- Adjunct vaginal lactic acid with single-dose metronidazole for bacterial vaginosis: a randomized controlled trial.BMC women's health · 2026Trial
- Phenotypic, genomic, and functional characterization of Corynebacterium sp. nov. isolated by droplet-based cultivation from vaginal swabs of a preeclampsia patient.BMC microbiology · 2026Article
- Addition of lactic acid bacteria to diluted ram semen as vehicle for vaginal inoculation: interaction with seminal microbiota, sperm quality and antibacterial in vitro effect against Mycoplasma agalactiae.BMC veterinary research · 2026Article
- The vital role of Lactobacillus sp. in vaginal health: Implications for enhanced prophylactic research.Probiotics and antimicrobial proteins · 2026Review
- Molecular Testing for the Diagnosis of Bacterial Vaginosis.International journal of molecular sciences · 2023Review
- Isolation and Identification of Lactic Acid Bacteria from Natural Whey Cultures of Buffalo and Cow Milk.Foods (Basel, Switzerland) · 2022Article
- Isolation, Identification, and Analysis of Potential Functions of Culturable Bacteria Associated with an Invasive Gall Wasp, Leptocybe invasa.Microbial ecology · 2022Article
- Microbiota identified from preserved Anopheles.Malaria journal · 2021Article
- Vaginal Microbiota Is Stable throughout the Estrous Cycle in Arabian Maress.Animals : an open access journal from MDPI · 2020Article
- Characterization of microbial communities in ethanol biorefineries.Journal of industrial microbiology & biotechnology · 2020Article
- Recent advances in cultivation-independent molecular-based techniques for the characterization of vaginal eubiosis and dysbiosis.Faculty reviews · 2020Review
- Article
- Vaginal microbiota and susceptibility to HIV.AIDS (London, England) · 2018Review
- Anaerobic Process for Bioenergy Recovery From Dairy Waste: Meta-Analysis and Enumeration of Microbial Community Related to Intermediates Production.Frontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
purposeTo date, molecular methods that circumvent the limitations of traditional culture methods have not been used to describe the vaginal microflora in India. Here, we compared culture and culture-independent molecular methods in characterizing the vaginal microbiota in Indian women. METHODOLOGY: Culture methods involved traditional cultivation on Rogosa and sheep blood agar, whereas culture-independent methods bypassed a culturing step by performing broadrange 16S rDNA PCR on DNA isolated directly from vaginal swabs.
resultsA total of 13 women were included in the study, of which five were characterized as healthy, two were bacterial vaginosis intermediate and six were bacterial vaginosis positive according to Nugent scoring. Lactobacillus jensenii was detected most frequently when using culture methods. On the other hand, Lactobacillus iners, which was not detected by culture methods, was the most common Lactobacillus sp. detected using cultivation-independent methods.
conclusionWe found little overlap between the species found using cultivation-dependent and cultivation-independent methods. Rather, culture-dependent and culture-independent methods were found to be complementary in describing the vaginal microflora among South Indian women. Culture-independent methods were found to be superior in detecting clinically relevant vaginal flora.
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