ArticleMolecular medicine reports2017
Estrogen stimulates osteoprotegerin expression via the suppression of miR-145 expression in MG-63 cells.
Article in Molecular medicine reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 47 citations in OpenAlex.
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- Pathophysiology and Molecular Signalling in Osteoporosis: Linking Risk Factors to Bone Loss.Journal of cellular and molecular medicine · 2026Review
- The obesity paradox in osteoporosis risk among older adults is mostly driven by women: a population-based prospective study.BMC geriatrics · 2025Article
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- Emerging Insights into the Endocrine Regulation of Bone Homeostasis by Gut Microbiome.Journal of the Endocrine Society · 2024Review
- MicroRNAs regulate the vicious cycle of vascular calcification-osteoporosis in postmenopausal women.Molecular biology reports · 2024Review
- MicroRNA Modulation during Orthodontic Tooth Movement: A Promising Strategy for Novel Diagnostic and Personalized Therapeutic Interventions.International journal of molecular sciences · 2022Review
- The Mechanism of Bone Remodeling After Bone Aging.Clinical interventions in aging · 2022Review
- The Emerging Role of MicroRNAs in Bone Diseases and Their Therapeutic Potential.Molecules (Basel, Switzerland) · 2021Review
- LncRNA ROR/miR-145-5p axis modulates the osteoblasts proliferation and apoptosis in osteoporosis.Bioengineered · 2021Article
- Circular RNA_0062582 promotes osteogenic differentiation of human bone marrow mesenchymal stem cells via regulation of microRNA-145/CBFB axis.Bioengineered · 2021Article
- Osteocytes and Estrogen Deficiency.Current osteoporosis reports · 2021Review
- Osteoprotegerin expression and serum values in obese women with type 2 diabetes mellitus.Molecular biology reports · 2021Article
- Gender-Related Differences in Chronic Kidney Disease-Associated Vascular Calcification Risk and Potential Risk Mediators: A Scoping Review.Healthcare (Basel, Switzerland) · 2021Article
- Upregulated osteoprotegerin expression promotes lung cancer cell invasion by increasing miR-20a expression.Experimental and therapeutic medicine · 2021Article
- Sex Differences in Proatherogenic Cytokine Levels.International journal of molecular sciences · 2020Article
- Osteoclastogenesis from bone marrow cells during estrogen-induced medullary bone formation in Japanese quails.Journal of molecular histology · 2019Article
- Compression and tension variably alter Osteoprotegerin expression via miR-3198 in periodontal ligament cells.BMC molecular and cell biology · 2019Article
- Estrogen deficiency impairs integrin αScientific reports · 2019Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Osteoprotegerin (OPG) is implicated in the pathogenesis of postmenopausal osteoporosis, and other metabolic bone diseases caused by estrogen deficiency. Previous studies have demonstrated that estrogen may stimulate OPG expression in osteoblast cells at the transcriptional level; however, whether estrogen can regulate OPG expression at a post-transcriptional level remains elusive. The present study aimed to investigate the role of microRNA (miRNA) in estrogen‑mediated OPG production in human osteoblast‑like MG‑63 cells. The results from ELISA, western blotting and reverse transcription-quantitative polymerase chain reaction (RT‑qPCR) confirmed that estrogen may upregulate OPG expression. Mechanistic studies indicated that estrogen increased the activity of a luciferase reporter harboring the OPG 3'‑untranslated region (3'‑UTR). Bioinformatics analysis demonstrated that there is a potential targeting site in the OPG 3'‑UTR for miRNA (miR)‑145, which is associated with osteoblast differentiation. The results of an RT‑qPCR suggested that estrogen suppressed miR‑145 expression. In addition, dual‑luciferase assay, RT‑qPCR and western blot analysis indicated that miR‑145 directly targets and negatively regulates OPG expression. Furthermore, transfection of cells with miR‑145 mimics was able to partially inhibit the induction of OPG expression by estrogen, thus confirming the role of miR‑145 in estrogen‑mediated OPG induction. Taken together, the results of the present study demonstrated that estrogen may post-transcriptionally regulate OPG expression through suppression of miR-145 expression.
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