ArticleJournal of structural biology2017
Monolayer-crystal streptavidin support films provide an internal standard of cryo-EM image quality.
Article in Journal of structural biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 41 citations in OpenAlex.
- Nickel-NTA lipid-monolayer affinity grids allow for high-resolution structure determination by cryo-EM.Journal of structural biology · 2025Article
- A novel "bio-tag" for cryo-EM studies based on the small, electron-dense protein Csp1.Biophysical journal · 2025Article
- RNA sample optimization for cryo-EM analysis.Nature protocols · 2025Review
- Activation of automethylated PRC2 by dimerization on chromatin.Molecular cell · 2024Article
- Fabrication of Monolayer Graphene-Coated Grids for Cryoelectron Microscopy.Journal of visualized experiments : JoVE · 2023Article
- A 3D-printed flow-cell for on-grid purification of electron microscopy samples directly from lysate.Journal of structural biology · 2023Article
- Polyelectrolyte coating of cryo-EM grids improves lateral distribution and prevents aggregation of macromolecules.Acta crystallographica. Section D, Structural biology · 2022Article
- Developing Graphene Grids for Cryoelectron Microscopy.Frontiers in molecular biosciences · 2022Review
- Perspective: Biochemical and Physical Constraints Associated With Preparing Thin Specimens for Single-Particle Cryo-EM.Frontiers in molecular biosciences · 2022Article
- Affinity Capture of p97 with Small-Molecule Ligand Bait Reveals a 3.6 Å Double-Hexamer Cryoelectron Microscopy Structure.ACS nano · 2021Article
- From Tube to Structure: SPA Cryo-EM Workflow Using Apoferritin as an Example.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Present and Emerging Methodologies in Cryo-EM Single-Particle Analysis.Biophysical journal · 2020Review
- What Could Go Wrong? A Practical Guide to Single-Particle Cryo-EM: From Biochemistry to Atomic Models.Journal of chemical information and modeling · 2020Article
- Sub-2 Angstrom resolution structure determination using single-particle cryo-EM at 200 keV.Journal of structural biology: X · 2020Article
- Cryo_fit: Democratization of flexible fitting for cryo-EM.Journal of structural biology · 2019Article
- 3.1 Å structure of yeast RNA polymerase II elongation complex stalled at a cyclobutane pyrimidine dimer lesion solved using streptavidin affinity grids.Journal of structural biology · 2019Article
- Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution.Nature communications · 2019Article
- Article
- Nanoscale mosaicity revealed in peptide microcrystals by scanning electron nanodiffraction.Communications biology · 2019Article
- A simple and robust procedure for preparing graphene-oxide cryo-EM grids.Journal of structural biology · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Analysis of images of biotinylated Escherichia coli 70S ribosome particles, bound to streptavidin affinity grids, demonstrates that the image-quality of particles can be predicted by the image-quality of the monolayer crystalline support film. The quality of the Thon rings is also a good predictor of the image-quality of particles, but only when images of the streptavidin crystals extend to relatively high resolution. When the estimated resolution of streptavidin was 5Å or worse, for example, the ribosomal density map obtained from 22,697 particles went to only 9.5Å, while the resolution of the map reached 4.0Å for the same number of particles, when the estimated resolution of streptavidin crystal was 4Å or better. It thus is easy to tell which images in a data set ought to be retained for further work, based on the highest resolution seen for Bragg peaks in the computed Fourier transforms of the streptavidin component. The refined density map obtained from 57,826 particles obtained in this way extended to 3.6Å, a marked improvement over the value of 3.9Å obtained previously from a subset of 52,433 particles obtained from the same initial data set of 101,213 particles after 3-D classification. These results are consistent with the hypothesis that interaction with the air-water interface can damage particles when the sample becomes too thin. Streptavidin monolayer crystals appear to provide a good indication of when that is the case.
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Registered trials
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