ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2017
Pitfalls in interpretation of CT-values of RT-PCR in children with acute respiratory tract infections.
Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Systematic review on the association between respiratory virus real-time PCR cycle threshold values and clinical presentation or outcomes.The Journal of antimicrobial chemotherapy · 2021Pooled it
- Nationwide population-level influenza cycle threshold values and trends in influenza incidence: a longitudinal study.Scientific reports · 2025Article
- Using Minimally Invasive Tissue Sampling and Determination of Cause of Death to Establish Etiologies of Community Respiratory Deaths Among Zambian Infants and Children.Journal of the Pediatric Infectious Diseases Society · 2025Article
- Occurrence of Central Nervous System Complications of Respiratory Syncytial Virus Infections: A Systematic Review with Meta-Analysis.Epidemiologia (Basel, Switzerland) · 2024Review
- Impact of Nirsevimab Immunization on Pediatric Hospitalization Rates: A Systematic Review and Meta-Analysis (2024).Vaccines · 2024Review
- Review
- Pathogenic Role of Human Rhinovirus Mono-Infection in Pediatric Lower Respiratory Tract Infection.Cureus · 2024Article
- Clinical Outcomes of Acutely Ill Children According to Cycle Threshold Values of Respiratory Viruses Detected by Multiplex PCR Testing.Journal of the Pediatric Infectious Diseases Society · 2023Article
- Article
- Interactions among 17 respiratory pathogens: a cross-sectional study using clinical and community surveillance data.medRxiv : the preprint server for health sciences · 2022Article
- Nitrogen Accumulation in Oyster (Life (Basel, Switzerland) · 2021Article
- A discussion of syndromic molecular testing for clinical care.The Journal of antimicrobial chemotherapy · 2021Article
- Caution in interpretation of SARS-CoV-2 quantification based on RT-PCR cycle threshold value.Diagnostic microbiology and infectious disease · 2021Article
- Viral Dynamics and Real-Time RT-PCR Ct Values Correlation with Disease Severity in COVID-19.Diagnostics (Basel, Switzerland) · 2021Review
- Observational
- Article
- Cycle Threshold Values in the Context of Multiple RT-PCR Testing for SARS-CoV-2.Risk management and healthcare policy · 2021Article
- Detection of SARS-CoV-2 in nasal swabs using MALDI-MS.Nature biotechnology · 2020Article
- A Systematic Review of the Clinical Utility of Cycle Threshold Values in the Context of COVID-19.Infectious diseases and therapy · 2020Review
- A large effective population size for established within-host influenza virus infection.eLife · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe relation between viral load and disease severity in childhood acute respiratory tract infections (ARI) is not fully understood.
objectivesTo assess the clinical relevance of the relation between viral load, determined by cycle threshold (CT) value of real-time reverse transcription-polymerase chain reaction assays and disease severity in children with single- and multiple viral ARI. STUDY
design582 children with ARI were prospectively followed and tested for 15 viruses. Correlations were calculated between CT values and clinical parameters.
resultsIn single viral ARI, statistically significant correlations were found between viral loads of Respiratory Syncytial Virus (RSV) and hospitalization and between viral loads of Human Coronavirus (HCoV) and a disease severity score. In multiple-viral ARI, statistically significant correlations between viral load and clinical parameters were found. In RSV-Rhinovirus (RV) multiple infections, a low viral load of RV was correlated with a high length of hospital stay and a high duration of extra oxygen use. The mean CT value for RV, HCoV and Parainfluenza virus was significantly lower in single- versus multiple infections.
conclusionAlthough correlations between CT values and clinical parameters in patients with single and multiple viral infection were found, the clinical importance of these findings is limited because individual differences in host-, viral and laboratory factors complicate the interpretation of statistically significant findings. In multiple infections, viral load cannot be used to differentiate between disease causing virus and innocent bystanders.
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