ArticlePlant molecular biology2017
Isolation and functional characterization of a methyl jasmonate-responsive 3-carene synthase from Lavandula x intermedia.
Article in Plant molecular biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Systematic engineering to enhance 3-carene production in yeast.Engineering microbiology · 2026Article
- Construction of Simple Sequence Repeat-Based Genetic Linkage Map and Identification of QTLs for Accumulation of Floral Volatiles in Lavender (International journal of molecular sciences · 2025Article
- Multi-omics analyses provide insights into the evolutionary history and the synthesis of medicinal components of the Chinese wingnut.Plant diversity · 2024Article
- Metabolic engineering of terpene metabolism in lavender.Beni-Suef University journal of basic and applied sciences · 2024Review
- Lavandula Species, Their Bioactive Phytochemicals, and Their Biosynthetic Regulation.International journal of molecular sciences · 2023Review
- Expression Profile and Ligand Screening of a Putative Odorant-Binding Protein, AcerOBP6, from the Asian Honeybee.Insects · 2021Article
- Article
- Short-chain isoprenyl diphosphate synthases of lavender (Lavandula).Plant molecular biology · 2020Article
- Diverse transcription factors control monoterpene synthase expression in lavender (Lavandula).Planta · 2019Article
- RNA-Seq in the discovery of a sparsely expressed scent-determining monoterpene synthase in lavender (Lavandula).Planta · 2019Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageA methyl jasmonate responsive 3-carene synthase (Li3CARS) gene was isolated from Lavandula x intermedia and functionally characterized in vitro. Lavenders produce essential oils consisting mainly of monoterpenes, including the potent antimicrobial and insecticidal monoterpene 3-carene. In this study we isolated and functionally characterized a leaf-specific, methyl jasmonate (MeJA)-responsive monoterpene synthase (Li3CARS) from Lavandula x intermedia. The ORF excluding transit peptides encoded a 64.9 kDa protein that was expressed in E. coli, and purified with Ni-NTA agarose affinity chromatography. The recombinant Li3CARS converted GPP into 3-carene as the major product, with K
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