ArticlePlant molecular biology2017
CRISPR/Cas9-mediated targeted mutagenesis in upland cotton (Gossypium hirsutum L.).
Article in Plant molecular biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Progress in genetic engineering and genome editing of peanuts: revealing the future of crop improvement.Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Review
- gRNA Design: How Its Evolution Impacted on CRISPR/Cas9 Systems Refinement.Biomolecules · 2023Review
- A comprehensive overview of cotton genomics, biotechnology and molecular biological studies.Science China. Life sciences · 2023Review
- Insights to Gossypium defense response against Verticillium dahliae: the Cotton Cancer.Functional & integrative genomics · 2023Review
- Exploring the Agrobacterium-mediated transformation with CRISPR/Cas9 in cucumber (Cucumis sativus L.).Molecular biology reports · 2022Article
- CRISPR/Cas9-Mediated Mutagenesis of the Granule-Bound Starch Synthase Gene in the Potato Variety Yukon Gold to Obtain Amylose-Free Starch in Tubers.International journal of molecular sciences · 2022Article
- Article
- Targeted plant improvement through genome editing: from laboratory to field.Plant cell reports · 2021Review
- Biofuels and Sustainability.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Genome-wide quantitative trait loci reveal the genetic basis of cotton fibre quality and yield-related traits in a Gossypium hirsutum recombinant inbred line population.Plant biotechnology journal · 2020Article
- Review
- Efficient genome editing of Brassica campestris based on the CRISPR/Cas9 system.Molecular genetics and genomics : MGG · 2019Article
- CRISPR/Cas9-Based Mutagenesis of Starch Biosynthetic Genes in Sweet Potato (Ipomoea Batatas) for the Improvement of Starch Quality.International journal of molecular sciences · 2019Article
- Establishment of Efficient Genetic Transformation Systems and Application of CRISPR/Cas9 Genome Editing Technology inInternational journal of molecular sciences · 2019Article
- Highly Efficient Targeted Gene Editing in Upland Cotton Using the CRISPR/Cas9 System.International journal of molecular sciences · 2018Article
- A transient transformation system for gene characterization in upland cotton (Plant methods · 2018Article
- Simultaneous Editing of Two Copies ofFrontiers in plant science · 2018Article
- CRISPR for Crop Improvement: An Update Review.Frontiers in plant science · 2018Review
- Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression.Plant methods · 2018Article
- Application of CRISPR/Cas9 Genome Editing Technology for the Improvement of Crops Cultivated in Tropical Climates: Recent Progress, Prospects, and Challenges.Frontiers in plant science · 2018Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR associated (Cas)9 protein system has emerged as a simple and efficient tool for genome editing in eukaryotic cells. It has been shown to be functional in several crop species, yet there are no reports on the application of this or any other genome editing technologies in the cotton plant. Cotton is an important crop that is grown mainly for its fiber, but its seed also serves as a useful source of edible oil and feed protein. Most of the commercially-grown cotton is tetraploid, thus making it much more difficult to target both sets of homeologous alleles. Therefore, in order to understand the efficacy of the CRISPR/Cas9 system to target a gene within the genome of cotton, we made use of a transgenic cotton line previously generated in our laboratory that had a single copy of the green fluorescent protein (GFP) gene integrated into its genome. We demonstrate, for the first time, the use of this powerful new tool in targeted knockout of a gene residing in the cotton genome. By following the loss of GFP fluorescence, we were able to observe the cells that had undergone targeted mutations as a result of CRISPR/Cas9 activity. In addition, we provide examples of the different types of indels obtained by Cas9-mediated cleavage of the GFP gene, guided by three independent sgRNAs. The results provide useful information that will help us target important native genes in the cotton plant in future.
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