ArticleCell stress & chaperones2017
LEA proteins are involved in cyst desiccation resistance and other abiotic stresses in Azotobacter vinelandii.
Article in Cell stress & chaperones, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 37 citations in OpenAlex.
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- Surviving desiccation: key factors underlying tolerance in prokaryotes and eukaryotes.Protoplasma · 2025Review
- Ammonia-oxidizing archaea and bacteria differentially contribute to ammonia oxidation in soil under precipitation gradients and land legacy.bioRxiv : the preprint server for biology · 2023Article
- Artificial Proteins Designed from G3LEA Contribute to Enhancement of Oxidation Tolerance inAntioxidants (Basel, Switzerland) · 2023Article
- The Azotobacter vinelandii AlgU regulon during vegetative growth and encysting conditions: A proteomic approach.PloS one · 2023Article
- Genome-wide identification and expression analysis of late embryogenesis abundant (Forestry research · 2023Article
- Regulation of the regulators: Transcription factors controlling biosynthesis of plant secondary metabolites during biotic stresses and their regulation by miRNAs.Frontiers in plant science · 2023Review
- Characterization of a NovelFrontiers in plant science · 2022Article
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- Xerotolerance: A New Property inMicroorganisms · 2021Article
- Late embryogenesis abundant (LEA) gene family inPlant signaling & behavior · 2021Article
- Nitrogen FixingFrontiers in microbiology · 2021Review
- Transcriptomic Analysis of the Grapevine LEA Gene Family in Response to Osmotic and Cold Stress Reveals a Key Role for VamDHN3.Plant & cell physiology · 2020Article
- Genome-wide identification of and functional insights into the late embryogenesis abundant (LEA) gene family in bread wheat (Triticum aestivum).Scientific reports · 2019Article
- Structural properties and cellular expression of AfrLEA6, a group 6 late embryogenesis abundant protein from embryos of Artemia franciscana.Cell stress & chaperones · 2019Article
- Overexpression of OsLea14-A improves the tolerance of rice and increases Hg accumulation under diverse stresses.Environmental science and pollution research international · 2019Article
- Cotton Late Embryogenesis Abundant (G3 (Bethesda, Md.) · 2018Article
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3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Late embryogenesis abundant (LEA) proteins constitute a large protein family that is closely associated with resistance to abiotic stresses in multiple organisms and protect cells against drought and other stresses. Azotobacter vinelandii is a soil bacterium that forms desiccation-resistant cysts. This bacterium possesses two genes, here named lea1 and lea2, coding for avLEA1 and avLEA2 proteins, both containing 20-mer motifs characteristic of eukaryotic plant LEA proteins. In this study, we found that disruption of the lea1 gene caused a loss of the cysts' viability after 3 months of desiccation, whereas at 6 months, wild-type or lea2 mutant strain cysts remained viable. Vegetative cells of the lea1 mutant were more sensitive to osmotic stress; cysts developed by this mutant were also more sensitive to high temperatures than cysts or vegetative cells of the wild type or of the lea2 mutant. Expression of lea1 was induced several fold during encystment. In addition, the protective effects of these proteins were assessed in Escherichia coli cells. We found that E. coli cells overexpressing avLEA1 were more tolerant to salt stress than control cells; finally, in vitro analysis showed that avLEA1 protein was able to prevent the freeze thaw-induced inactivation of lactate dehydrogenase. In conclusion, avLEA1 is essential for the survival of A. vinelandii in dry conditions and for protection against hyper-osmolarity, two major stress factors that bacteria must cope with for survival in the environment. This is the first report on the role of bacterial LEA proteins on the resistance of cysts to desiccation.
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