ArticleNature communications2017
Rational design of inducible CRISPR guide RNAs for de novo assembly of transcriptional programs.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed.
- 3D Genome Engineering Using CRISPR/dCas Systems.International journal of molecular sciences · 2026Review
- Gene therapy: The current trajectory of clinical and commercial translation.Progress in biophysics and molecular biology · 2026Review
- A programmable ribozyme for RNA signal transduction.Nature communications · 2026Article
- Conditional guide RNA deactivation by mRNA and small molecule triggers in Saccharomyces cerevisiae.New biotechnology · 2025Article
- Oligo-CALL: A next-generation barcoding platform for studying resistance to targeted therapy.Science advances · 2025Article
- Article
- Enhancing RNA editing efficiency and specificity with engineered ADAR2 guide RNAs.Molecular therapy. Nucleic acids · 2025Article
- Tissue-specific modulation of CRISPR activity by miRNA-sensing guide RNAs.Nucleic acids research · 2025Article
- Logical regulation of endogenous gene expression using programmable, multi-input processing CRISPR guide RNAs.Nucleic acids research · 2024Article
- Establishing artificial gene connections through RNA displacement-assembly-controlled CRISPR/Cas9 function.Nucleic acids research · 2023Article
- Biosensor-assisted titratable CRISPRi high-throughput (BATCH) screening for over-production phenotypes.Metabolic engineering · 2023Article
- RNA-Responsive gRNAs for Controlling CRISPR Activity: Current Advances, Future Directions, and Potential Applications.The CRISPR journal · 2022Review
- CRISPR signal conductor 2.0 for redirecting cellular information flow.Cell discovery · 2022Article
- Controlling CRISPR with small molecule regulation for somatic cell genome editing.Molecular therapy : the journal of the American Society of Gene Therapy · 2022Review
- Review
- Regulating CRISPR/Cas9 Function through Conditional Guide RNA Control.Chembiochem : a European journal of chemical biology · 2021Review
- Controlling and enhancing CRISPR systems.Nature chemical biology · 2021Review
- Conditional guide RNA through two intermediate hairpins for programmable CRISPR/Cas9 function: building regulatory connections between endogenous RNA expressions.Nucleic acids research · 2020Article
- CRISPR-based gene expression control for synthetic gene circuits.Biochemical Society transactions · 2020Review
- Photocontrol of CRISPR/Cas9 function by site-specific chemical modification of guide RNA.Chemical science · 2020Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
CRISPR-based transcription regulators (CRISPR-TRs) have transformed the current synthetic biology landscape by allowing specific activation or repression of any target gene. Here we report a modular and versatile framework enabling rapid implementation of inducible CRISPR-TRs in mammalian cells. This strategy relies on the design of a spacer-blocking hairpin (SBH) structure at the 5' end of the single guide RNA (sgRNA), which abrogates the function of CRISPR-transcriptional activators. By replacing the SBH loop with ligand-controlled RNA-cleaving units, we demonstrate conditional activation of quiescent sgRNAs programmed to respond to genetically encoded or externally delivered triggers. We use this system to couple multiple synthetic and endogenous target genes with specific inducers, and assemble gene regulatory modules demonstrating parallel and orthogonal transcriptional programs. We anticipate that this 'plug and play' approach will be a valuable addition to the synthetic biology toolkit, facilitating the understanding of natural gene circuits and the design of cell-based therapeutic strategies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.