ArticleScientific reports2017
Multimodal discrimination of immune cells using a combination of Raman spectroscopy and digital holographic microscopy.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 52 citations in OpenAlex.
- From spectroscopic analysis to clinical imaging: emerging applications and persistent challenges of raman spectroscopy in CTCs-based liquid biopsies.Annals of medicine · 2026Review
- Raman spectroscopy combined with multiple technologies for label-free identification of immune cells: An overview.Journal of pharmaceutical analysis · 2026Review
- Measurement of HbA1c with a new phase function using RBC phase image from a low-cost digital holographic microscopy.Biotechnology letters · 2025Article
- Characterization of a Single-Capture Bright-Field and Off-Axis Digital Holographic Microscope for Biological Applications.Sensors (Basel, Switzerland) · 2025Article
- Correlative Raman Imaging: Development and Cancer Applications.Biosensors · 2024Review
- Investigation of refractive index dynamics duringBiomedical optics express · 2023Article
- In vivo optical molecular imaging of inflammation and immunity.Journal of molecular medicine (Berlin, Germany) · 2021Review
- Decoding Optical Data with Machine Learning.Laser & photonics reviews · 2021Article
- Raman Microscopy: Progress in Research on Cancer Cell Sensing.Sensors (Basel, Switzerland) · 2020Review
- Combined Raman and polarization sensitive holographic imaging for a multimodal label-free assessment of human sperm function.Scientific reports · 2019Article
- Towards automated cancer screening: Label-free classification of fixed cell samples using wavelength modulated Raman spectroscopy.Journal of biophotonics · 2018Article
- Noninvasive detection of macrophage activation with single-cell resolution through machine learning.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Raman Plus X: Biomedical Applications of Multimodal Raman Spectroscopy.Sensors (Basel, Switzerland) · 2017Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to identify and characterise individual cells of the immune system under label-free conditions would be a significant advantage in biomedical and clinical studies where untouched and unmodified cells are required. We present a multi-modal system capable of simultaneously acquiring both single point Raman spectra and digital holographic images of single cells. We use this combined approach to identify and discriminate between immune cell populations CD4+ T cells, B cells and monocytes. We investigate several approaches to interpret the phase images including signal intensity histograms and texture analysis. Both modalities are independently able to discriminate between cell subsets and dual-modality may therefore be used a means for validation. We demonstrate here sensitivities achieved in the range of 86.8% to 100%, and specificities in the range of 85.4% to 100%. Additionally each modality provides information not available from the other providing both a molecular and a morphological signature of each cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.