Evidence map›Paper›PMID 28256545›Full record

ArticleScientific reports2017

The influence of haemoglobin and iron on in vitro mycobacterial growth inhibition assays.

Rachel Tanner, Matthew K O'Shea, Andrew D White, Julius Müller, Rachel Harrington-Kandt, Magali Matsumiya, Mike J Dennis, Eneida A Parizotto, Stephanie Harris, Elena Stylianou and 6 more

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 23 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed, 3 pooled it
3.1field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 3 syntheses or guidelines pooled it, 33 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Anemia, Iron Status, and HIV: A Systematic Review of the Evidence.Advances in nutrition (Bethesda, Md.) · 2020
    Pooled it
  4. Trial
  5. Trial
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Review
  13. TheF1000Research · 2021
    Article
  14. Article
  15. Article
  16. Frontiers in immunology · 2019
    Article
  17. Article
  18. Article
  19. Article
  20. Frontiers in immunology · 2018
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

16 authors at 4 institutions in 1 country.

Rachel TannerThe Jenner Institute, University of Oxford, Oxford, UK.
Matthew K O'SheaThe Jenner Institute, University of Oxford, Oxford, UK.
Andrew D WhitePublic Health England, Porton Down, Salisbury, UK.
Julius MüllerThe Jenner Institute, University of Oxford, Oxford, UK.
Rachel Harrington-KandtThe Jenner Institute, University of Oxford, Oxford, UK.
Magali MatsumiyaThe Jenner Institute, University of Oxford, Oxford, UK.
Mike J DennisPublic Health England, Porton Down, Salisbury, UK.
Eneida A ParizottoThe Jenner Institute, University of Oxford, Oxford, UK.
Stephanie HarrisThe Jenner Institute, University of Oxford, Oxford, UK.
Elena StylianouThe Jenner Institute, University of Oxford, Oxford, UK.
Vivek NaranbhaiThe Jenner Institute, University of Oxford, Oxford, UK.
Paulo BettencourtThe Jenner Institute, University of Oxford, Oxford, UK.
Hal DrakesmithWeatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK.
Sally SharpePublic Health England, Porton Down, Salisbury, UK.
Helen A FletcherLondon School of Hygiene and Tropical Medicine, London, UK.
Helen McShaneThe Jenner Institute, University of Oxford, Oxford, UK.
Jenner Institute · GBUniversity of Oxford · GBPublic Health England · GBLondon School of Hygiene & Tropical Medicine · GB

Funding

Medical Research Council MC_UU_12010/10Wellcome Trust
6 · The paper itself

Abstract

The current vaccine against tuberculosis, live attenuated Mycobacterium bovis BCG, has variable efficacy, but development of an effective alternative is severely hampered by the lack of an immune correlate of protection. There has been a recent resurgence of interest in functional in vitro mycobacterial growth inhibition assays (MGIAs), which provide a measure of a range of different immune mechanisms and their interactions. We identified a positive correlation between mean corpuscular haemoglobin and in vitro growth of BCG in whole blood from healthy UK human volunteers. Mycobacterial growth in peripheral blood mononuclear cells (PBMC) from both humans and macaques was increased following the experimental addition of haemoglobin (Hb) or ferric iron, and reduced following addition of the iron chelator deferoxamine (DFO). Expression of Hb genes correlated positively with mycobacterial growth in whole blood from UK/Asian adults and, to a lesser extent, in PBMC from South African infants. Taken together our data indicate an association between Hb/iron levels and BCG growth in vitro, which may in part explain differences in findings between whole blood and PBMC MGIAs and should be considered when using such assays.

Indexed as

AdolescentAdultAnimalsBCG VaccineDeferoxamineErythrocyte IndicesGene ExpressionHemoglobinsHumansInfantIronIron Chelating AgentsLeukocytes, MononuclearLymphocyte ActivationMacaca mulattaMicrobial Sensitivity TestsBCG VaccineDeferoxamineHemoglobinsIronIron Chelating Agents

Identifiers

PMID28256545
PMCPMC5335253
OpenAlexW2594664021

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.