ArticleBiophysical journal2017
Viscoelastic Properties of Confluent MDCK II Cells Obtained from Force Cycle Experiments.
Article in Biophysical journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 74 citations in OpenAlex.
- Mapping single-cell rheology of ascidian embryos in the cleavage stages using AFM.Biophysical journal · 2025Article
- Tissue tension of planar, free standing cell monolayers measured by central deformation.PNAS nexus · 2025Article
- Extracellular Matrix Viscoelasticity: A Dynamic Regulator of Cellular Behavior.Annals of biomedical engineering · 2025Review
- Multiscale mechanical-adapted hydrogels for the repair of intervertebral disc degeneration.Bioactive materials · 2025Article
- Article
- Effects of vimentin on the migration, search efficiency, and mechanical resilience of dendritic cells.Biophysical journal · 2022Article
- Epithelial cells sacrifice excess area to preserve fluidity in response to external mechanical stress.Communications biology · 2022Article
- Foregut organ progenitors and their niche display distinct viscoelastic properties in vivo during early morphogenesis stages.Communications biology · 2022Article
- An explicit model to extract viscoelastic properties of cells from AFM force-indentation curves.iScience · 2022Article
- The Role of Cytoskeleton Revealed by Quartz Crystal Microbalance and Digital Holographic Microscopy.International journal of molecular sciences · 2022Article
- Epithelial cells fluidize upon adhesion but display mechanical homeostasis in the adherent state.Biophysical journal · 2022Article
- Viscoelasticity of basal plasma membranes and cortices derived from MDCK II cells.Biophysical reports · 2021Article
- Viscoelasticity and Noise Properties Reveal the Formation of Biomemory in Cells.The journal of physical chemistry. B · 2021Article
- The nature of cell division forces in epithelial monolayers.The Journal of cell biology · 2021Article
- Human mammary epithelial cells in a mature, stratified epithelial layer flatten and stiffen compared to single and confluent cells.Biochimica et biophysica acta. General subjects · 2021Article
- Multi-oscillation microrheologyLab on a chip · 2021Article
- Buckling of an Epithelium Growing under Spherical Confinement.Developmental cell · 2020Article
- Viscoelasticity in simple indentation-cycle experiments: a computational study.Scientific reports · 2020Article
- Mechanical Characterization for Cellular Mechanobiology: Current Trends and Future Prospects.Frontiers in bioengineering and biotechnology · 2020Review
- Stiffness of MDCK II Cells Depends on Confluency and Cell Size.Biophysical journal · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The local mechanical properties of cells are frequently probed by force indentation experiments carried out with an atomic force microscope. Application of common contact models provides a single parameter, the Young's modulus, to describe the elastic properties of cells. The viscoelastic response of cells, however, is generally measured in separate microrheological experiments that provide complex shear moduli as a function of time or frequency. Here, we present a straightforward way to obtain rheological properties of cells from regular force distance curves collected in typical force indentation measurements. The method allows us to record the stress-strain relationship as well as changes in the weak power law of the viscoelastic moduli. We derive an analytical function based on the elastic-viscoelastic correspondence principle applied to Hertzian contact mechanics to model both indentation and retraction curves. Rheological properties are described by standard viscoelastic models and the paradigmatic weak power law found to interpret the viscoelastic properties of living cells best. We compare our method with atomic force microscopy-based active oscillatory microrheology and show that the method to determine the power law coefficient is robust against drift and largely independent of the indentation depth and indenter geometry. Cells were subject to Cytochalasin D treatment to provoke a drastic change in the power law coefficient and to demonstrate the feasibility of the approach to capture rheological changes extremely fast and precisely. The method is easily adaptable to different indenter geometries and acquires viscoelastic data with high spatiotemporal resolution.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.