ArticleCell and tissue banking2017
Cell lines authentication and mycoplasma detection as minimun quality control of cell lines in biobanking.
Article in Cell and tissue banking, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 48 citations in OpenAlex.
- A novel and simple workflow for investigating Mycoplasma spp. contamination in cell cultures.Folia microbiologica · 2025Article
- Authentication of RBK (Razi Bovine Kidney) Cell Line Derived from Primary Kidney Cell.Archives of Razi Institute · 2025Article
- Detection of viral contamination in cell lines using ViralCellDetector.Frontiers in microbiology · 2025Article
- Single-cell transcriptome conservation in a multispecies comparative analysis of fresh and cryopreserved insulinoma cell lines.Veterinary oncology (London, England) · 2025Article
- Unlocking Potential: A Comprehensive Overview of Cell Culture Banks and Their Impact on Biomedical Research.Cells · 2024Review
- Preclinical Mouse Metastatic Model Established Through Induced Lung Metastases.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Application of RapidHIT™ ID for cell authentication by fast and convenient STR profiling.Genes & genomics · 2023Article
- Development and Evaluation of a New qPCR Assay for the Detection ofCurrent issues in molecular biology · 2023Article
- Identification of cell culture contamination by an unusual species ofCytotechnology · 2023Article
- Genetic Characterization of Rat Hepatic Stellate Cell Line HSC-T6 for In Vitro Cell Line Authentication.Cells · 2022Article
- A Clonogenic Assay to Quantify Melanoma Micrometastases in Pulmonary Tissue.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Biobanks-A Platform for Scientific and Biomedical Research.Diagnostics (Basel, Switzerland) · 2020Review
- Keeping It Clean: The Cell Culture Quality Control Experience at the National Center for Advancing Translational Sciences.SLAS discovery : advancing life sciences R & D · 2020Article
- Mycoplasma Infection Mediates Sensitivity of Multidrug-Resistant Cell Lines to Tiopronin: A Cautionary Tale.Journal of medicinal chemistry · 2020Article
- Long-Term Stability and Differentiation Potential of Cryopreserved cGMP-Compliant Human Induced Pluripotent Stem Cells.International journal of molecular sciences · 2019Article
- Biobanking in health care: evolution and future directions.Journal of translational medicine · 2019Review
- The human microglial HMC3 cell line: where do we stand? A systematic literature review.Journal of neuroinflammation · 2018Review
- Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines.Journal of extracellular vesicles · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Establishment of continuous cell lines from human normal and tumor tissues is an extended and useful methodology for molecular characterization of cancer pathophysiology and drug development in research laboratories. The exchange of these cell lines between different labs is a common practice that can compromise assays reliability due to contamination with microorganism such as mycoplasma or cells from different flasks that compromise experiment reproducibility and reliability. Great proportions of cell lines are contaminated with mycoplasma and/or are replaced by cells derived for a different origin during processing or distribution process. The scientific community has underestimated this problem and thousand of research experiment has been done with cell lines that are incorrectly identified and wrong scientific conclusions have been published. Regular contamination and authentication tests are necessary in order to avoid negative consequences of widespread misidentified and contaminated cell lines. Cell banks generate, store and distribute cell lines for research, being mandatory a consistent and continuous quality program. Methods implementation for guaranteeing both, the absence of mycoplasma and authentication in the supplied cell lines, has been performed in the Andalusian Health System Biobank. Specifically, precise results were obtained using real time PCR detection for mycoplasma and 10 STRs identification by capillary electrophoresis for cell line authentication. Advantages and disadvantages of these protocols are discussed.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.