ArticleMethods in molecular biology (Clifton, N.J.)2017
Reconstitution of TCR Signaling Using Supported Lipid Bilayers.
Article in Methods in molecular biology (Clifton, N.J.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 37 citations in OpenAlex.
- Hyperactive PLCG1 induces cell-autonomous and bystander T cell activation and drug resistance.EMBO reports · 2025Article
- Design of sensitive monospecific and bispecific synthetic chimeric T cell receptors for cancer therapy.Nature cancer · 2025Article
- Protein interactions, calcium, phosphorylation, and cholesterol modulate CFTR cluster formation on membranes.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Functional differences between rodent and human PD-1 linked to evolutionary divergence.Science immunology · 2025Article
- Prior Fc receptor activation primes macrophages for increased sensitivity to IgG via long-term and short-term mechanisms.Developmental cell · 2024Article
- Phosphorylation-regulated phase separation of syndecan-4 and syntenin promotes the biogenesis of exosomes.Cell proliferation · 2024Article
- The physical landscape of CAR-T synapse.Biophysical journal · 2024Review
- Sensitive bispecific chimeric T cell receptors for cancer therapy.Research square · 2024Article
- Two-dimensional molecular condensation in cell signaling and mechanosensing.Acta biochimica et biophysica Sinica · 2023Review
- Leveraging DNA Origami to Study Phagocytosis.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Biomolecular Condensation of SH2 Domain-Containing Proteins on Membranes.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Recruitment and organization of ESCRT-0 and ubiquitinated cargo via condensation.Science advances · 2022Article
- Receptor tyrosine kinases regulate signal transduction through a liquid-liquid phase separated state.Molecular cell · 2022Article
- Phase separation in immune signalling.Nature reviews. Immunology · 2022Review
- Synergistic phase separation of two pathways promotes integrin clustering and nascent adhesion formation.eLife · 2022Article
- Reconstitution of Phase-Separated Signaling Clusters and Actin Polymerization on Supported Lipid Bilayers.Frontiers in cell and developmental biology · 2022Article
- Membrane-associated phase separation: organization and function emerge from a two-dimensional milieu.Journal of molecular cell biology · 2021Review
- Xanthomonas effector XopR hijacks host actin cytoskeleton via complex coacervation.Nature communications · 2021Article
- PLCγ1 promotes phase separation of T cell signaling components.The Journal of cell biology · 2021Article
- Retromer forms low order oligomers on supported lipid bilayers.The Journal of biological chemistry · 2020Article
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 1 country.
Funding
Abstract
Biochemical reconstitution has served as an important tool for understanding the mechanisms of many cellular processes including DNA replication, transcription, translation, vesicle trafficking, and ubiquitin-mediated proteolysis. Here, we demonstrate that biochemical reconstitution can be applied to studying a complex signaling pathway involving as many as 12 proteins or protein complexes acting at the surface of model membranes. We show that a temporal sequence of events in activated T cells beginning with phosphorylation of the T cell receptor and culminating in the activation of actin polymerization can be replicated in vitro. Our reconstitution demonstrates the sufficiency of these proteins in producing many of the complex behaviors observed during T cell activation. The ability to manipulate all of the components, measure reaction rates, and observe molecular behaviors, including at single molecule resolution, has enabled us to gain insight into some of the important biochemical features of this signaling pathway such as microcluster formation. The same system could be adapted to study other membrane-proximal signaling pathways, including growth factor receptors, death receptors, and Eph receptors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.