Evidence map›Paper›PMID 28255693›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2017

Analyzing Actin Dynamics at the Immunological Synapse.

Katarzyna I Jankowska, Janis K Burkhardt

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
12.1field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
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  3. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Katarzyna I JankowskaDepartment of Pathology and Laboratory Medicine, Children's Hospital of Philadelphia Research Institute, 3615 Civic Center Blvd, ARC 816D, Philadelphia, PA, 19104, USA.
Janis K BurkhardtDepartment of Pathology and Laboratory Medicine, Children's Hospital of Philadelphia Research Institute, 3615 Civic Center Blvd, ARC 816D, Philadelphia, PA, 19104, USA. jburkhar@mail.med.upenn.edu.
Children's Hospital of Philadelphia · US

Funding

Crosstalk between T cells and inflamed endothelium: regulation by Crk family proteinsR01HL128551 · NHLBI · CHILDREN'S HOSP OF PHILADELPHIA · PI BURKHARDT, JANIS K. · 2015 to 2018
$1.7M
Costimulatory ligand mobility effects on T cell activationR01GM104867 · NIGMS · CHILDREN'S HOSP OF PHILADELPHIA · PI BURKHARDT, JANIS K. · 2013 to 2016
$1.3M
NHLBI NIH HHS R01 HL128551NIGMS NIH HHS R01 GM104867
6 · The paper itself

Abstract

T cell signaling is inextricably linked to actin cytoskeletal dynamics at the immunological synapse (IS). This process can be imaged in living T cells expressing GFP actin or fluorescent F-actin binding proteins. Because of its planar nature, the IS provides a unique opportunity to image events as they happen, monitoring changes in actin retrograde flow in T cells interacting with different stimulatory surfaces or after pharmacological treatments. Here, we described the imaging methods and analytical procedures used to measure actin velocity across the IS in T cells spreading on planar stimulatory surfaces.

Indexed as

ActinsAnimalsGreen Fluorescent ProteinsHumansImmunological SynapsesJurkat CellsMiceT-LymphocytesActinsGreen Fluorescent ProteinsActinCytoskeletonImmunological synapseIntegrinKymographLive cell imagingMobile ligandsPlanar lipid bilayerSpinning diskT-cells

Identifiers

PMID28255693
PMCPMC6126369
OpenAlexW2592904451

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.