Evidence map›Paper›PMID 28255013›Full record

ArticleHaematologica2017

Histone deacetylase 6 regulates cytokinesis and erythrocyte enucleation through deacetylation of formin protein mDia2.

Xuehui Li, Yang Mei, Bowen Yan, Eric Vitriol, Suming Huang, Peng Ji, Yi Qiu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Haematologica, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 34 citations in OpenAlex.

  1. Article
  2. Article
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  4. Article
  5. Erythroblast enucleation at a glance.Journal of cell science · 2024
    Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Review
  11. Review
  12. Article
  13. Analyses of erythropoiesis from embryonic stem cell-CD34Journal of cellular and molecular medicine · 2022
    Article
  14. Review
  15. Article
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  17. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Xuehui LiDepartment of Anatomy and Cell Biology, College of Medicine, University of Florida, Gainesville, FL, USA.
Yang MeiDepartment of Pathology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.
Bowen YanDepartment of Anatomy and Cell Biology, College of Medicine, University of Florida, Gainesville, FL, USA.
Eric VitriolDepartment of Anatomy and Cell Biology, College of Medicine, University of Florida, Gainesville, FL, USA.
Suming HuangDepartment of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, FL, USA.
Peng JiDepartment of Pathology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA qiuy@ufl.edu peng-ji@fsm.northwestern.edu.
Yi QiuDepartment of Anatomy and Cell Biology, College of Medicine, University of Florida, Gainesville, FL, USA qiuy@ufl.edu peng-ji@fsm.northwestern.edu.
University of Florida · USNorthwestern University · USQuality Research · US

Funding

The roles of pleckstrin-2 as a functional node in erythropoiesisR01DK102718 · NIDDK · NORTHWESTERN UNIVERSITY AT CHICAGO · PI JI, PENG · 2015 to 2019
$1.7M
The role of HDAC1 acetylation on corepressor complex activity and hematopoiesisR01HL095674 · NHLBI · UNIVERSITY OF FLORIDA · PI QIU, YI · 2010 to 2013
$1.4M
The Role of Long Noncoding RNA in HematopoiesisR01DK110108 · NIDDK · UNIVERSITY OF FLORIDA · PI HUANG, SUMING · 2016 to 2019
$1.3M
Novel mechanisms of actin dynamics underlying cell motility, axon growth, and ALSR00NS087104 · NINDS · UNIVERSITY OF FLORIDA · PI VITRIOL, ERIC A · 2015 to 2017
$736k
NHLBI NIH HHS R01 HL095674NIDDK NIH HHS R01 DK102718NIDDK NIH HHS R01 DK110108NINDS NIH HHS R00 NS087104
6 · The paper itself

Abstract

The formin protein mDia2 plays a critical role in a number of cellular processes through its ability to promote nucleation and elongation of actin filaments. In erythroblasts, this includes control of cytokinesis and enucleation by regulating contractile actin ring formation. Here we report a novel mechanism of how mDia2 is regulated: through acetylation and deacetylation at lysine 970 in the formin homology 2 domain. Ectopic expression of an acetyl-mimic mDia2 mutant in mouse erythroblasts is sufficient to abolish contractile actin ring formation at the cleavage furrow and subsequent erythrocyte cytokinesis and enucleation. We also identified that class II histone deacetylase 6 deacetylates and subsequently activates mDia2. Knockdown or inhibition of histone deacetylase 6 impairs contractile actin ring formation, and expression of a non-acetyl-mimic mDia2 mutant restores the contractile actin ring and rescues the impairment of enucleation. In addition to revealing a new step in mDia2 regulation, this study may unveil a novel regulatory mechanism of formin-mediated actin assembly, since the K970 acetylation site is conserved among Dia proteins.

Indexed as

CytokinesisErythropoiesisAcetylationActinsAnimalsCells, CulturedErythroblastsHistone Deacetylase 6LysineMiceMicrofilament ProteinsMicrotubule-Associated ProteinsNADPH DehydrogenaseActinsDia2 protein, mouseHistone Deacetylase 6LysineMicrofilament ProteinsMicrotubule-Associated ProteinsNADPH Dehydrogenase

Identifiers

PMID28255013
PMCPMC5451330
OpenAlexW2592722317

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.