ArticleMicrobiome2017
Simple discovery of bacterial biocatalysts from environmental samples through functional metaproteomics.
Article in Microbiome, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- Unlocking Enzyme Discovery: Leveraging Multi-Omics, Machine Learning, and De Novo Design.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Culture-independent discovery of a novel thermotolerant lipase and its producer from mesophilic anaerobic digestion sludge.Applied microbiology and biotechnology · 2025Article
- Activity- and Enrichment-Based Metaproteomics Insights into Active Urease from the Rumen Microbiota of Cattle.International journal of molecular sciences · 2022Article
- A roadmap for metagenomic enzyme discovery.Natural product reports · 2021Review
- A multi-omic screening approach for the discovery of thermoactive glycoside hydrolases.Extremophiles : life under extreme conditions · 2021Article
- Metaproteomic Discovery and Characterization of a Novel Lipolytic Enzyme From an Indian Hot Spring.Frontiers in microbiology · 2021Article
- Soil Metaproteomics for the Study of the Relationships Between Microorganisms and Plants: A Review of Extraction Protocols and Ecological Insights.International journal of molecular sciences · 2020Review
- The Holo-Transcriptome of the ZoantharianMarine drugs · 2018Article
- Characterization of ML-005, a Novel Metaproteomics-Derived Esterase.Frontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Bacterial biocatalysts play a key role in our transition to a bio-based, post-petroleum economy. However, the discovery of new biocatalysts is currently limited by our ability to analyze genomic information and our capacity of functionally screening for desired activities. Here, we present a simple workflow that combines functional metaproteomics and metagenomics, which facilitates the unmediated and direct discovery of biocatalysts in environmental samples. To identify the entirety of lipolytic biocatalysts in a soil sample contaminated with used cooking oil, we detected all proteins active against a fluorogenic substrate in sample's metaproteome using a 2D-gel zymogram. Enzymes' primary structures were then deduced by tryptic in-gel digest and mass spectrometry of the active protein spots, searching against a metagenome database created from the same contaminated soil sample. We then expressed one of the novel biocatalysts heterologously in Escherichia coli and obtained proof of lipolytic activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.