Evidence map›Paper›PMID 28253888›Full record

ArticleJournal of translational medicine2017

Stability of pro- and anti-inflammatory immune biomarkers for human cohort studies.

C Graham, R Chooniedass, W P Stefura, L Lotoski, P Lopez, A D Befus, A B Becker, K T HayGlass

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of translational medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
1.2field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 39 citations in OpenAlex.

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  5. Associations of Individual and Combined Physical Activity and Body Mass Index Groups with Proinflammatory Biomarkers among Colorectal Cancer Patients.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

C GrahamDepartment of Immunology, University of Manitoba, Winnipeg, MB, Canada.
R ChooniedassDepartment of Pediatrics and Child Health, University of Manitoba, Winnipeg, MB, Canada.
W P StefuraDepartment of Immunology, University of Manitoba, Winnipeg, MB, Canada.
L LotoskiDepartment of Immunology, University of Manitoba, Winnipeg, MB, Canada.
P LopezDepartment of Immunology, University of Manitoba, Winnipeg, MB, Canada.
A D BefusDivision of Pulmonary Medicine, Department of Medicine, University of Alberta, Edmonton, AB, Canada.
A B BeckerDepartment of Immunology, University of Manitoba, Winnipeg, MB, Canada.
K T HayGlassDepartment of Immunology, University of Manitoba, Winnipeg, MB, Canada. kent.hayglass@umanitoba.ca.
University of Manitoba · CAUniversity of Alberta · CA

Funding

CIHR
6 · The paper itself

Abstract

backgroundAlthough discovery research has identified the importance of dozens of pro- and anti-inflammatory immune mediators in the pathogenesis, maintenance, exacerbation and resolution of inflammatory diseases, most human cohort studies have incorporated few or no immunological intermediate phenotypes in their analyses. Significant hindrances have been (1) the limited panel of biomarkers known to be readily detected in healthy human populations and (2) the stability, hence utility, of such biomarkers to repeated analysis.

methodsThe frequency and stability of 14 plasma biomarkers linked to in vivo immune regulation of allergic and autoimmune inflammatory disorders was determined in 140 healthy pediatric and adult participants. The impact of initial and multiple subsequent freeze/thaw cycles on pro-inflammatory (CCL2, CXCL10, IL-18, TNFα, IL-6), anti-inflammatory (IL-10, sTNF-RII, IL-1Ra), acute phase proteins (CRP, PTX3) and other biomarkers (sST2, IL-1RAcP) was subsequently quantified.

resultsMultiple biomarkers capable of providing an innate immune signature of inflammation were readily detected directly ex vivo in healthy individuals. These biomarker levels were unaffected when comparing paired data sets from freshly obtained, never frozen plasma or serum and matched aliquots despite extensive freeze/thaw cycles. Neither age nor sex affected stability. Similarly, no quantitative differences were found following repetitive analysis of inflammatory biomarkers in culture samples obtained following in vitro stimulation with TLR and RLR ligands.

conclusionsA broad panel of in vivo and ex vivo cytokine, chemokine and acute phase protein biomarkers that have been linked to human chronic inflammatory disorders are readily detected in vivo and remain stable for analysis despite multiple freeze thaw cycles. These data provide the foundation and confidence for large scale analyses of panels of inflammatory biomarkers to provide better understanding of immunological mechanisms underlying health versus disease.

Indexed as

Anti-Inflammatory AgentsBiomarkersCells, CulturedCohort StudiesFemaleFreezingHumansInflammation MediatorsMaleSerumTissue DonorsAnti-Inflammatory AgentsBiomarkersInflammation MediatorsAllergyAutoimmunityCohortHuman immunologyImmune regulationInflammationInnate

Identifiers

PMID28253888
PMCPMC5335797
OpenAlexW2593746507

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.