ArticleScientific reports2017
Automated sub-5 nm image registration in integrated correlative fluorescence and electron microscopy using cathodoluminescence pointers.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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Who cites it
10 citing papers in PubMed.
- Traceable localization enables accurate integration of quantum emitters and photonic structures with high yield.Optica quantum · 2024Article
- A workflow for streamlined acquisition and correlation of serial regions of interest in array tomography.BMC biology · 2021Article
- Optimization of negative stage bias potential for faster imaging in large-scale electron microscopy.Journal of structural biology: X · 2021Article
- Integrated Array Tomography for 3D Correlative Light and Electron Microscopy.Frontiers in molecular biosciences · 2021Article
- AutoCLEM: An Automated Workflow for Correlative Live-Cell Fluorescence Microscopy and Cryo-Electron Tomography.Scientific reports · 2019Article
- Applications, promises, and pitfalls of deep learning for fluorescence image reconstruction.Nature methods · 2019Review
- An Easy Path for Correlative Electron and Super-Resolution Light Microscopy.Scientific reports · 2019Article
- Towards robust and versatile single nanoparticle fiducial markers for correlative light and electron microscopy.Journal of microscopy · 2019Article
- The 2018 correlative microscopy techniques roadmap.Journal of physics D: Applied physics · 2018Review
- Automated detection of fluorescent cells in in-resin fluorescence sections for integrated light and electron microscopy.Journal of microscopy · 2018Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the biological sciences, data from fluorescence and electron microscopy is correlated to allow fluorescence biomolecule identification within the cellular ultrastructure and/or ultrastructural analysis following live-cell imaging. High-accuracy (sub-100 nm) image overlay requires the addition of fiducial markers, which makes overlay accuracy dependent on the number of fiducials present in the region of interest. Here, we report an automated method for light-electron image overlay at high accuracy, i.e. below 5 nm. Our method relies on direct visualization of the electron beam position in the fluorescence detection channel using cathodoluminescence pointers. We show that image overlay using cathodoluminescence pointers corrects for image distortions, is independent of user interpretation, and does not require fiducials, allowing image correlation with molecular precision anywhere on a sample.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.