ArticleScientific reports2017
Prevention of Staphylococcus aureus biofilm formation by antibiotics in 96-Microtiter Well Plates and Drip Flow Reactors: critical factors influencing outcomes.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
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- Differential antimicrobial responses in polymicrobial triple-species biofilms associated with persistent urinary tract infections.Scientific reports · 2026Article
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- Development of Martian saline seep models and their implications for planetary protection.Biofilm · 2023Article
- Effect of N-Acetyl-L-cysteine on Activity of Doxycycline against Biofilm-Forming Bacterial Strains.Antibiotics (Basel, Switzerland) · 2023Article
- Phage and Antibiotic Combinations ReduceViruses · 2023Article
- Antimicrobial Treatment ofAntibiotics (Basel, Switzerland) · 2023Review
- Innovative Perspectives on Biofilm Interactions in Poultry Drinking Water Systems and Veterinary Antibiotics Used Worldwide.Antibiotics (Basel, Switzerland) · 2022Article
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- In Vitro Antimicrobial Susceptibility Testing of Biofilm-Growing Bacteria: Current and Emerging Methods.Advances in experimental medicine and biology · 2022Review
- New Adapted In Vitro Technology to Evaluate Biofilm Formation and Antibiotic Activity Using Live Imaging under Flow Conditions.Diagnostics (Basel, Switzerland) · 2021Article
- Characterizing the Shearing Stresses within the CDC Biofilm Reactor Using Computational Fluid Dynamics.Microorganisms · 2021Article
- Experimental infection of Asian house geckos with Enterococcus lacertideformus demonstrates multiple disease transmission routes and the in-vivo efficacy of antibiotics.Scientific reports · 2021Article
- Multisubstituted pyrimidines effectively inhibit bacterial growth and biofilm formation of Staphylococcus aureus.Scientific reports · 2021Article
- Optimization of a High-Throughput 384-Well Plate-Based Screening Platform withInternational journal of molecular sciences · 2020Article
- Article
- Structural and Functional Dynamics ofMicroorganisms · 2019Article
- Biofilm Formation byJournal of clinical medicine · 2019Article
- Methods Used for the Eradication of Staphylococcal Biofilms.Antibiotics (Basel, Switzerland) · 2019Review
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Biofilm formation leads to the failure of antimicrobial therapy. Thus, biofilm prevention is a desirable goal of antimicrobial research. In this study, the efficacy of antibiotics (doxycycline, oxacillin and rifampicin) in preventing Staphylococcus aureus biofilms was investigated using Microtiter Well Plates (MWP) and Drip Flow Reactors (DFR), two models characterized by the absence and the presence of a continuous flow of nutrients, respectively. Planktonic culture of S. aureus was exposed to antibiotics for one hour followed by 24 hours incubation with fresh nutrients in MWP or continuous flow of nutrients in DFR. The DFR grown biofilms were significantly more tolerant to the antibiotics than those grown in MWP without the continuous flow. The differences in log reductions (LR) between the two models could not be attributed to differences in the cell density, the planktonic inoculum concentration or the surface-area-to-volume ratios. However, eliminating the flow in the DFR significantly restored the antibiotic susceptibility. These findings demonstrate the importance of considering differences between experimental conditions in different model systems, particularly the flow of nutrients, when performing anti-biofilm efficacy evaluations. Biofilm antibiotic efficacy studies should be assessed using various models and more importantly, in a model mimicking conditions of its clinical application.
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