ArticleScientific reports2017
Revealing the sequence of interactions of PuroA peptide with Candida albicans cells by live-cell imaging.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- Membrane-Targeted Consequences of Acetaminophen Toxicity and Off-Target Effects of Antimicrobial Peptides on Host Cell Membranes.International journal of molecular sciences · 2026Review
- The antimicrobial peptide EM86 loaded to gamma-irradiated sodium alginate/polyvinyl alcohol electrospun nanofibrous dressing treated multidrug-resistantFrontiers in bioengineering and biotechnology · 2026Article
- Hundreds of antimicrobial peptides create a selective barrier for insect gut symbionts.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Fast killing kinetics, significant therapeutic index, and high stability of melittin-derived antimicrobial peptide.Amino acids · 2022Article
- Cecropin D-derived synthetic peptides in the fight againstFrontiers in microbiology · 2022Article
- Physicochemical Features and Peculiarities of Interaction of AMP with the Membrane.Pharmaceuticals (Basel, Switzerland) · 2021Review
- Effects of Rationally Designed Physico-Chemical Variants of the Peptide PuroA on Biocidal Activity towards Bacterial and Mammalian Cells.International journal of molecular sciences · 2020Article
- Spectroscopic and Microscopic Approaches for Investigating the Dynamic Interactions of Anti-microbial Peptides With Membranes and Cells.Frontiers in medical technology · 2020Review
- De Novo Design and In Vitro Testing of Antimicrobial Peptides against Gram-Negative Bacteria.Pharmaceuticals (Basel, Switzerland) · 2019Article
- The antimicrobial properties of the puroindolines, a review.World journal of microbiology & biotechnology · 2019Review
- Antimicrobial Efficacy of Indolicidin Against Multi-Drug Resistant EnteroaggregativeFrontiers in microbiology · 2019Article
- Physico-Chemical and Antifungal Properties of a Trypsin Inhibitor from the Roots ofMolecules (Basel, Switzerland) · 2018Article
- Antimicrobial peptides: biochemical determinants of activity and biophysical techniques of elucidating their functionality.World journal of microbiology & biotechnology · 2018Review
- Live-cell Imaging of Fungal Cells to Investigate Modes of Entry and Subcellular Localization of Antifungal Plant Defensins.Journal of visualized experiments : JoVE · 2017Article
- Fluorescence lifetime imaging ophthalmoscopy.Progress in retinal and eye research · 2017Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To determine the mechanism(s) of action of antimicrobial peptides (AMPs) it is desirable to provide details of their interaction kinetics with cellular, sub-cellular and molecular targets. The synthetic peptide, PuroA, displays potent antimicrobial activities which have been attributed to peptide-induced membrane destabilization, or intracellular mechanisms of action (DNA-binding) or both. We used time-lapse fluorescence microscopy and fluorescence lifetime imaging microscopy (FLIM) to directly monitor the localization and interaction kinetics of a FITC- PuroA peptide on single Candida albicans cells in real time. Our results reveal the sequence of events leading to cell death. Within 1 minute, FITC-PuroA was observed to interact with SYTO-labelled nucleic acids, resulting in a noticeable quenching in the fluorescence lifetime of the peptide label at the nucleus of yeast cells, and cell-cycle arrest. A propidium iodide (PI) influx assay confirmed that peptide translocation itself did not disrupt the cell membrane integrity; however, PI entry occurred 25-45 minutes later, which correlated with an increase in fractional fluorescence of pores and an overall loss of cell size. Our results clarify that membrane disruption appears to be the mechanism by which the C. albicans cells are killed and this occurs after FITC-PuroA translocation and binding to intracellular targets.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.