ArticleScientific reports2017
A Novel Impedimetric Microfluidic Analysis System for Transgenic Protein Cry1Ab Detection.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 20 citations in OpenAlex.
- A Review on Microfluidics-Based Impedance Biosensors.Biosensors · 2023Review
- Enhancing the performance of paper-based electrochemical impedance spectroscopy nanobiosensors: An experimental approach.Biosensors & bioelectronics · 2021Article
- Aptamer-Modified Magnetic Beads in Biosensing.Sensors (Basel, Switzerland) · 2018Review
- Microfluidic methods for aptamer selection and characterization.The Analyst · 2017Review
- Bioelectrochemical Detection of Mycobacterium tuberculosis ESAT-6 in an Antibody-Based Biomicrosystem.Sensors (Basel, Switzerland) · 2017Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Impedimetric analysis method is an important tool for food safety detection. In this work, a novel impedimetric microfluidic analysis system consisted of a printed gold electrode chip and a microfluidic flow cell was developed for sensitive and selective detection of transgenic protein Cry1Ab. Anti-Cry1Ab aptamer coated magnetic beads were used to recognize transgenic protein Cry1Ab and form Cry1Ab-aptamer modified magnetic beads. After separation, the obtained Cry1Ab-aptamer modified magnetic beads were dissolved in 0.01 M mannitol and followed by injection into the microfluidic flow cell for impedimetric measurement. At the frequency of 358.3 Hz, the impedance signal shows a good linearity with the concentrations of Cry1Ab protein at a range from 0 to 0.2 nM, and the detection limit is 0.015 nM. The results demonstrate that the impedimetric microfluidic analysis system provides an alternative way to enable sensitive, rapid and specific detection of transgenic protein Cry1Ab.
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