Evidence map›Paper›PMID 28251925›Full record

ArticleMolecular biology of the cell2017

The RhoGAP protein ARHGAP18/SENEX localizes to microtubules and regulates their stability in endothelial cells.

Michael D Lovelace, Elizabeth E Powter, Paul R Coleman, Yang Zhao, Amelia Parker, Garry H Chang, Angelina J Lay, Julie Hunter, Aaron P McGrath, Mika Jormakka and 5 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular biology of the cell, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
3.7field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 22 citations in OpenAlex.

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  14. Super-resolution microscopy demystified.Nature cell biology · 2019
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 2 institutions in 1 country.

Michael D LovelaceCentre for the Endothelium, Vascular Biology Program, University of Sydney, Sydney, NSW 2050, Australia.
Elizabeth E PowterCentre for the Endothelium, Vascular Biology Program, University of Sydney, Sydney, NSW 2050, Australia.
Paul R ColemanCentre for the Endothelium, Vascular Biology Program, University of Sydney, Sydney, NSW 2050, Australia.
Yang ZhaoCentre for the Endothelium, Vascular Biology Program, University of Sydney, Sydney, NSW 2050, Australia.
Amelia ParkerTumour Biology and Targeting Program, Children's Cancer Institute, Lowy Cancer Research Centre, University of New South Wales, Sydney, NSW 2052, Australia.
Garry H ChangCentre for the Endothelium, Vascular Biology Program, University of Sydney, Sydney, NSW 2050, Australia.
Angelina J LayCentre for the Endothelium, Vascular Biology Program, University of Sydney, Sydney, NSW 2050, Australia.
Julie HunterCentre for the Endothelium, Vascular Biology Program, University of Sydney, Sydney, NSW 2050, Australia.
Aaron P McGrathStructural Biology Laboratory, University of Sydney, Sydney, NSW 2050, Australia.
Mika JormakkaStructural Biology Laboratory, University of Sydney, Sydney, NSW 2050, Australia.
Patrick BertolinoLiver Immunology Laboratory, University of Sydney, Sydney, NSW 2050, Australia.
Geoffrey McCaughanLiver Biology and Cancer Laboratory, Centenary Institute, University of Sydney, Sydney, NSW 2050, Australia.
Maria KavallarisTumour Biology and Targeting Program, Children's Cancer Institute, Lowy Cancer Research Centre, University of New South Wales, Sydney, NSW 2052, Australia.
Mathew A VadasCentre for the Endothelium, Vascular Biology Program, University of Sydney, Sydney, NSW 2050, Australia j.gamble@centenary.org.au m.vadas@centenary.org.au.
Jennifer R GambleCentre for the Endothelium, Vascular Biology Program, University of Sydney, Sydney, NSW 2050, Australia j.gamble@centenary.org.au m.vadas@centenary.org.au.
The University of Sydney · AUCancer Institute of New South Wales · AU

Funding

Ferroportin Structure and functionR01DK107309 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI JORMAKKA, MIKA, MACKENZIE, BRYAN · 2015 to 2019
$3.0M
NIDDK NIH HHS R01 DK107309
6 · The paper itself

Abstract

RhoGTPases are important regulators of the cell cytoskeleton, controlling cell shape, migration and proliferation. Previously we showed that ARHGAP18 in endothelial cells is important in cell junctions. Here we show, using structured illumination microscopy (SIM), ground-state depletion (GSD), and total internal reflection fluorescence microscopy (TIRF) that a proportion of ARHGAP18 localizes to microtubules in endothelial cells, as well as in nonendothelial cells, an association confirmed biochemically. In endothelial cells, some ARHGAP18 puncta also colocalized to Weibel-Palade bodies on the microtubules. Depletion of ARHGAP18 by small interfering RNA or analysis of endothelial cells isolated from ARHGAP18-knockout mice showed microtubule destabilization, as evidenced by altered morphology and decreased acetylated α-tubulin and glu-tubulin. The destabilization was rescued by inhibition of ROCK and histone deacetylase 6 but not by a GAP-mutant form of ARHGAP18. Depletion of ARHGAP18 resulted in a failure to secrete endothelin-1 and a reduction in neutrophil transmigration, both known to be microtubule dependent. Thrombin, a critical regulator of the Rho-mediated barrier function of endothelial cells through microtubule destabilization, enhanced the plasma membrane-bound fraction of ARHGAP18. Thus, in endothelial cells, ARHGAP18 may act as a significant regulator of vascular homeostasis.

Indexed as

AcetylationActinsAnimalsCell MovementCells, CulturedCytoskeletonEndothelial CellsGTPase-Activating ProteinsHeLa CellsHistone Deacetylase 6Histone DeacetylasesHumansHuman Umbilical Vein Endothelial CellsIntercellular JunctionsMiceMice, KnockoutActinsARHGAP18 protein, humanARHGAP18 protein, mouseGTPase-Activating ProteinsHDAC6 protein, humanHistone Deacetylase 6Histone Deacetylasesrho-Associated KinasesTubulin

Identifiers

PMID28251925
PMCPMC5391183
OpenAlexW2591878032

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.