ArticleCell biology and toxicology2017
A fish intestinal epithelial barrier model established from the rainbow trout (Oncorhynchus mykiss) cell line, RTgutGC.
Article in Cell biology and toxicology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 103 citations in OpenAlex.
- From immature to mature epithelium: unveiling structural dynamics and transcriptional programs in rainbow trout intestinal barrier.Veterinary research · 2026Article
- Coexposure of Micro and Nano-Plastics with Pesticides: Cytotoxicity and Bioaccumulation Effects on a Fish Intestinal Cell Line.Environmental science & technology · 2026Article
- Multiomics profiling of zebrafish embryonic cell line PAC2 across growth phases to assess its relevance for toxicological studies.PloS one · 2026Article
- A systematic approach towards long-term, serum-free cultivation of fish cells with the RTgill-W1 cell line as example.NAM journal · 2025Article
- Functional feed ingredients modulate the immune response of RTgutGC cells to LPS-induced inflammation.Frontiers in immunology · 2025Article
- Making in vitro conditions more reflective of in vivo conditions for research on the teleost gastrointestinal tract.The Journal of experimental biology · 2024Review
- Metabolic disrupting chemicals in the intestine: the need for biologically relevant models: Zebrafish: what can we learn from this small environment-sensitive fish?FEBS open bio · 2024Review
- Role of UV radiation and oxidation on polyethylene micro- and nanoplastics: impacts on cadmium sorption, bioaccumulation, and toxicity in fish intestinal cells.Environmental science and pollution research international · 2024Article
- Fish cell line: depositories, web resources and future applications.Cytotechnology · 2024Review
- In vitro fish mucosal surfaces producing mucin as a model for studying host-pathogen interactions.PloS one · 2024Article
- The effects ofFrontiers in immunology · 2024Article
- Effectiveness of functional ingredients to enhance gill disease in Atlantic salmon (Salmo salar, L.).PloS one · 2024Article
- Article
- Development of a Rainbow Trout (Animals : an open access journal from MDPI · 2023Article
- Establishment of an In Vitro Model to Study Viral Infections of the Fish Intestinal Epithelium.Cells · 2023Article
- Bioaccessibility of Organic Compounds Associated with Tire Particles Using a FishEnvironmental science & technology · 2022Article
- Assessment of Conventional and Low Gossypol Cottonseed Meal as Alternative Protein Sources in Low-Fishmeal Diets of Hybrid Grouper (Animals : an open access journal from MDPI · 2022Article
- Determination of the Dissolution/Permeation and Apparent Solubility for Microencapsulated Emamectin Benzoate Using In Vitro and Ex VivoPharmaceuticals (Basel, Switzerland) · 2022Article
- Effect of chloride concentration on the cytotoxicity, bioavailability, and bioreactivity of copper and silver in the rainbow trout gut cell line, RTgutGC.Ecotoxicology (London, England) · 2022Article
- Role and relevance of fish cell lines in advanced in vitro research.Molecular biology reports · 2022Review
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The intestine of fish is a multifunctional organ: lined by only a single layer of specialized epithelial cells, it has various physiological roles including nutrient absorption and ion regulation. It moreover comprises an important barrier for environmental toxicants, including metals. Thus far, knowledge of the fish intestine is limited largely to in vivo or ex vivo investigations. Recently, however, the first fish intestinal cell line, RTgutGC, was established, originating from a rainbow trout (Oncorhynchus mykiss). In order to exploit the opportunities arising from RTgutGC cells for exploring fish intestinal physiology and toxicology, we present here the establishment of cells on commercially available permeable membrane supports and evaluate its suitability as a model of polarized intestinal epithelia. Within 3 weeks of culture, RTgutGC cells show epithelial features by forming tight junctions and desmosomes between adjacent cells. Cells develop a transepithelial electrical resistance comparable to in vivo measured values, reflecting the leaky nature of the fish intestine. Immunocytochemistry reveals evidence of polarization, such as basolateral localization of Na
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.