ArticleCytotechnology2017
The enhancement of antibody concentration and achievement of high cell density CHO cell cultivation by adding nucleoside.
Article in Cytotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 47 citations in OpenAlex.
- Development and preliminary physicochemical characterization of a Ramucirumab biosimilar candidate in CHO cells using shake-flask fed-batch culture.Bioprocess and biosystems engineering · 2026Article
- Viable cell density as an indicator for dynamic feeding strategy in fed-batch and perfusion CHO cell culture.Scientific reports · 2025Article
- Development of an HEK293 Suspension Cell Culture Medium, Transient Transfection Optimization Workflow, and Analytics for Batch rAAV Manufacturing.Biotechnology and bioengineering · 2025Article
- Incorporating shaken 24-deep-well plate fed-batch culture shortens CHO cell line development time.Cytotechnology · 2025Article
- Enhancing monoclonal antibody production efficiency using CHO-MK cells and specific media in a conventional fed-batch culture.Cytotechnology · 2025Article
- NMR metabolomics of plant and yeast-based hydrolysates for cell culture media applications - A comprehensive assessment.Current research in food science · 2024Article
- Comparability strategy and demonstration for post-approval production cell line change of a bevacizumab biosimilar IBI305.Antibody therapeutics · 2023Article
- Rapid Identification of Chinese Hamster Ovary Cell Apoptosis and Its Potential Role in Process Robustness Assessment.Bioengineering (Basel, Switzerland) · 2023Article
- Progress in fed-batch culture for recombinant protein production in CHO cells.Applied microbiology and biotechnology · 2023Review
- Generation and Characterization of Stable Redox-Reporter Mammalian Cell Lines of Biotechnological Relevance.Sensors (Basel, Switzerland) · 2022Article
- Identification of novel inhibitory metabolites and impact verification on growth and protein synthesis in mammalian cells.Metabolic engineering communications · 2021Article
- A Metabolomics Approach to Increasing Chinese Hamster Ovary (CHO) Cell Productivity.Metabolites · 2021Article
- Rich production media as a platform for CHO cell line development.AMB Express · 2020Article
- Article
- Improvement of growth rates through nucleoside media supplementation of CHO clones.Cytotechnology · 2019Article
- Secretion analysis of intracellular "difficult-to-express" immunoglobulin G (IgG) in Chinese hamster ovary (CHO) cells.Cytotechnology · 2019Article
- Analysis of the immunoglobulin G (IgG) secretion efficiency in recombinant Chinese hamster ovary (CHO) cells by using Citrine-fusion IgG.Cytotechnology · 2019Article
- Article
- Protein hydrolysates in cell culture: Toward multi-omics characterization.Biotechnology progressReview
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recently, with the dramatic increase in demand for therapeutic antibodies, Chinese hamster ovary (CHO) cell culture systems have made significant progress in recombinant antibody production. Over the past two decades, recombinant antibody productivity has been improved by more than 100-fold. Medium optimization has been identified as an important key approach for increasing product concentrations. In this study, we evaluated the effects of deoxyuridine addition to fed-batch cultures of antibody-expressing CHO cell lines. Furthermore, we investigated the effects of combined addition of deoxyuridine, thymidine, and deoxycytidine. Our results suggest that addition of these pyrimidine nucleosides can increase CHO cell growth, with no significant change in the specific production rate. As a result of the increased cell growth, the antibody concentration was elevated and we were able to achieve more than 9 g/L during 16 days of culture. Similar effects of nucleoside addition were observed in fed-batch cultures of a Fab fragment-expressing CHO cell line, and the final Fab fragment concentration was more than 4 g/L. This nucleoside addition strategy could be a powerful platform for efficient antibody production.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.