ArticleEuropean biophysics journal : EBJ2017
Binding mechanism of PicoGreen to DNA characterized by magnetic tweezers and fluorescence spectroscopy.
Article in European biophysics journal : EBJ, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 20 citations in OpenAlex.
- Complete Pathway Refactoring and Combinatorial Biosynthesis of Anthracyclines for Modulation of Histone Eviction and DNA Damage Bioactivities.Angewandte Chemie (International ed. in English) · 2026Article
- Molecular structure and DNA binding mode of unsymmetric cyanine dyes RiboGreen and OliGreen.Nucleic acids research · 2026Article
- Design, Synthesis, and Evaluation of a Novel Phenanthrene Derivative as a Potential DNA Intercalator.ACS chemical biology · 2026Article
- Fluorescent Dyes for Differential Scanning Fluorimetry of RNA.Chembiochem : a European journal of chemical biology · 2025Article
- Effect of Molecular Weight of Fluorescent Dyes on DNA Separation by Capillary Electrophoresis.Journal of fluorescence · 2025Article
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- Comprehensive in vitro and in ovo assessment of cytotoxicity: Unraveling the impact of sodium fluoride, xylitol, and their synergistic associations in dental products.Biomolecules & biomedicine · 2024Article
- A novel method using a differential staining fluorescence microscopy (DSFM) to track the location of enteric pathogens within mixed-species biofilms.Scientific reports · 2023Article
- Photonics of Some Monomethine Cyanine Dyes in Solutions and in Complexes with Biomolecules.International journal of molecular sciences · 2023Article
- An analysis of interactions between three structurally diverse anthocyanidins, as well as their glucosides, and model biological membranes, albumin, and plasmid DNA.Scientific reports · 2023Article
- Inhibitors of ApiAP2 protein DNA binding exhibit multistage activity against Plasmodium parasites.PLoS pathogens · 2022Article
- Molecular structure, DNA binding mode, photophysical properties and recommendations for use of SYBR Gold.Nucleic acids research · 2021Article
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- Chromosome analysis and sorting.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2021Review
- Anticooperative Binding Governs the Mechanics of Ethidium-Complexed DNA.Biophysical journal · 2019Article
- Binding mechanism of anti-cancer chemotherapeutic drug mitoxantrone to DNA characterized by magnetic tweezers.Journal of nanobiotechnology · 2018Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescent dyes are broadly used in many biotechnological applications to detect and visualize DNA molecules. However, their binding to DNA alters the structural and nanomechanical properties of DNA and, thus, interferes with associated biological processes. In this work we employed magnetic tweezers and fluorescence spectroscopy to investigate the binding of PicoGreen to DNA at room temperature in a concentration-dependent manner. PicoGreen is an ultrasensitive quinolinium nucleic acid stain exhibiting hardly any background signal from unbound dye molecules. By means of stretching and overwinding single, torsionally constrained, nick-free double-stranded DNA molecules, we acquired force-extension and supercoiling curves which allow quantifying DNA contour length, persistence length and other thermodynamical binding parameters, respectively. The results of our magnetic tweezers single-molecule binding study were well supported through analyzing the fluorescent spectra of stained DNA. On the basis of our work, we could identify a concentration-dependent bimodal binding behavior, where, apparently, PicoGreen associates to DNA as an intercalator and minor-groove binder simultaneously.
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