ArticleNature methods2017
Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers.
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Article in Nature methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 105 papers.
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105citing papers in PubMed
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Who cites it
105 citing papers in PubMed.
- A simple and practical approach for protein serial crystallography using grease matrix and large-area support film.Journal of synchrotron radiation · 2026Article
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- Precisely Timed On-Demand Droplet Ejection and Collision in Free Space for Controlled Reaction Initiation.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- An additional water is introduced into the manganese cluster during the formation of the SNature communications · 2026Article
- High-yield charge separation along the alternative path in a photosynthetic reaction center: X-ray structure and electrostatic analysis.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Drop-on-fixed-target reaction initiation approach for serial and time-resolved crystallography.IUCrJ · 2026Article
- Tracking the redox reaction of the iron enzyme ribonucleotide reductase using continuous SerialED and SFX.Structure (London, England : 1993) · 2026Article
- Compact tape-driven sample delivery system for serial femtosecond crystallography.Journal of applied crystallography · 2026Article
- Article
- Structural basis of the promiscuity of the unusual Fe(II) and 2-oxoglutarate dependent human aspartate/asparagine-β-hydroxylase.Nature communications · 2026Article
- Can ferric-oxyl excited states explain elongated iron-oxygen bonds in heme peroxidase catalytic intermediates?Nature communications · 2026Article
- Microfluidic Devices for High-Throughput In Situ Serial Crystallography.Advances in biochemical engineering/biotechnology · 2026Review
- Advances in Crystallography and Cryo-Electron Microscopy for the Study of Membrane Proteins.Advances in experimental medicine and biology · 2026Review
- The Single-Particle, Clusters and Biomolecules and Serial Femtosecond Crystallography instrument of the European X-ray Free-Electron Laser: Interaction Region Downstream at atmospheric pressure (IRD).Journal of synchrotron radiation · 2026Article
- Sample delivery methods for protein X-ray crystallography with a special focus on sample consumption.Nature communications · 2025Review
- Combining Reactive Quantum-Mechanical Molecular-Dynamics Simulations with Mutagenesis, Crystallography, and Enzyme Kinetics to Reveal Plausible Steps of Isocyanide Hydratase Catalysis.Journal of chemical information and modeling · 2025Article
- Evolution of macromolecular crystallography beamlines at the Swiss Light Source and SwissFEL.Journal of synchrotron radiation · 2025Article
- Advancing time-resolved structural biology: latest strategies in cryo-EM and X-ray crystallography.Nature methods · 2025Review
- Advances in microfluidic mixers for time-resolved structural biology with X-rays.Biophysical reviews · 2025Review
- Serial-femtosecond crystallography reveals how a phytochrome variant couples chromophore and protein structural changes.Science advances · 2025Article
45 more citing papers are in PubMed but not listed here.
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Authors and funding
56 authors.
Franklin D FullerMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Sheraz GulMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Ruchira ChatterjeeMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
E Sethe BurgieDepartment of Biology, Washington University in St. Louis, St. Louis, Missouri, USA.
Iris D YoungMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Hugo LebretteDepartment of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.
Vivek SrinivasDepartment of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.
Aaron S BrewsterMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Tara Michels-ClarkMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Jonathan A ClingerDepartment of BioSciences, Rice University, Houston, Texas, USA.
Babak AndiNational Synchrotron Light Source II, Brookhaven National Laboratory, Upton, New York, USA.ORCID http://orcid.org/0000-0002-7666-639X
Mohamed IbrahimInstitut für Biologie, Humboldt-Universität zu Berlin, Berlin, Germany.
Ernest PastorMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Casper de LichtenbergInstitutionen för Kemi, Kemiskt Biologiskt Centrum, Umeå Universitet, Umeå, Sweden.
Rana HusseinInstitut für Biologie, Humboldt-Universität zu Berlin, Berlin, Germany.
Christopher J PollockDepartment of Chemistry, Pennsylvania State University, University Park, Pennsylvania, USA.
Miao ZhangInstitut für Biologie, Humboldt-Universität zu Berlin, Berlin, Germany.
Claudiu A StanStanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Thomas KrollSSRL, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Thomas FranssonStanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Clemens WeningerStanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Markus KubinInstitute for Methods and Instrumentation on Synchrotron Radiation Research, Helmholtz Zentrum Berlin für Materialien und Energie GmbH, Berlin, Germany.
Pierre AllerDiamond Light Source Limited, Harwell Science and Innovation Campus, Didcot, UK.
Louise LassalleMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Philipp BräuerDiamond Light Source Limited, Harwell Science and Innovation Campus, Didcot, UK.
Mitchell D MillerDepartment of BioSciences, Rice University, Houston, Texas, USA.
Muhamed AminMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Sergey KoroidovInstitutionen för Kemi, Kemiskt Biologiskt Centrum, Umeå Universitet, Umeå, Sweden.
Christian G RoesslerNational Synchrotron Light Source II, Brookhaven National Laboratory, Upton, New York, USA.
Marc AllaireMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Raymond G SierraLCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA.ORCID http://orcid.org/0000-0002-6253-8282
Peter T DockerDiamond Light Source Limited, Harwell Science and Innovation Campus, Didcot, UK.
James M GlowniaLCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Silke NelsonLCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Jason E KoglinLCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA.ORCID http://orcid.org/0000-0001-6811-6083
Diling ZhuLCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Matthieu CholletLCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Sanghoon SongLCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA.ORCID http://orcid.org/0000-0003-3493-1850
Henrik LemkeLCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA.ORCID http://orcid.org/0000-0003-1577-8643
Mengning LiangLCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Dimosthenis SokarasSSRL, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
Roberto Alonso-MoriLCLS, SLAC National Accelerator Laboratory, Menlo Park, California, USA.ORCID http://orcid.org/0000-0002-5357-0934
Athina ZouniInstitut für Biologie, Humboldt-Universität zu Berlin, Berlin, Germany.
Johannes MessingerInstitutionen för Kemi, Kemiskt Biologiskt Centrum, Umeå Universitet, Umeå, Sweden.
Uwe BergmannStanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.ORCID http://orcid.org/0000-0001-5639-166X
Amie K BoalDepartment of Chemistry, Pennsylvania State University, University Park, Pennsylvania, USA.
J Martin BollingerDepartment of Chemistry, Pennsylvania State University, University Park, Pennsylvania, USA.
Carsten KrebsDepartment of Chemistry, Pennsylvania State University, University Park, Pennsylvania, USA.
Martin HögbomDepartment of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.
George N PhillipsDepartment of BioSciences, Rice University, Houston, Texas, USA.
Richard D VierstraDepartment of Biology, Washington University in St. Louis, St. Louis, Missouri, USA.
Nicholas K SauterMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Allen M OrvilleDiamond Light Source Limited, Harwell Science and Innovation Campus, Didcot, UK.
Jan KernMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.ORCID http://orcid.org/0000-0002-7272-1603
Vittal K YachandraMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Junko YanoMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.ORCID http://orcid.org/0000-0001-6308-9071
Funding
X-RAY STRUCTURE OF RIFM PROTEIN FROM THE BIOSYNTHESIS PATHWAY OF THE ANSAMYCIN AP41RR012408 · NCRR · BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB · PI MARIUZZA, ROY A · 1998 to 2011
$32.2MX-ray Absorption Spectroscopy (XAS) pp.711-759P41GM103393 · NIGMS · STANFORD UNIVERSITY · PI HODGSON, KEITH O · 2012 to 2019
$30.6MLife Science and Biomedical technology Research center at NSLS-II LSBRP41GM111244 · NIGMS · BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB · PI MCSWEENEY, SEAN · 2014 to 2018
$16.4MTaking Snapshots of the Water-Oxidation Reaction by the Mn4Ca Oxygen-Evolving Complex Using X-ray Crystallography and SpectroscopyR01GM055302 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI YACHANDRA, VITTAL · 1997 to 2022
$9.1MHOUSTON AREA MOLECULAR BIOPHYSICS TRAINING PROGRAMT32GM008280 · NIGMS · RICE UNIVERSITY · PI WENSEL, THEODORE G · 1988 to 2023
$8.0MDIALS: New Computational Methods to Enable Challenging Crystallographic ExperimentsR01GM117126 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI SAUTER, NICHOLAS K · 2016 to 2023
$6.1MMacromolecular Crystallography at the National Synchrotron Light SourceP41GM103473 · NIGMS · BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB · PI SWEET, ROBERT M · 2012 to 2013
$4.9MBiological Spectroscopy and Crystallography Using an X-ray Free Electron Laser - Supplemental EquipmentR01GM110501 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI Junko Yano · 2014 to 2026
$4.8MTowards real-time XFEL data reduction with CCTBXR01GM102520 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI SAUTER, NICHOLAS K · 2012 to 2015
$1.4MSUPPORT FOR CONTINUOUS RESEARCH EXCELLENCES06GM008003 · NIGMS · SOUTHEASTERN OKLAHOMA STATE UNIVERSITY · PI PAIVA, NANCY · 1989 to 2007
$1.3MStructure and Function of the Mn Oxygen-Evolving ComplexR56GM055302 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI YACHANDRA, VITTAL · 2009 to 2009
$530kMechanistic Determination of the Non-heme Iron Enzyme Hyoscyamine-6-hydroxylaseF32GM113389 · NIGMS · PENNSYLVANIA STATE UNIVERSITY, THE · PI POLLOCK, CHRISTOPHER · 2015 to 2017
$160kNCRR NIH HHS P41 RR012408NIGMS NIH HHS F32 GM113389NIGMS NIH HHS F32 GM116423NIGMS NIH HHS P41 GM103393NIGMS NIH HHS P41 GM103473NIGMS NIH HHS P41 GM111244NIGMS NIH HHS R01 GM055302NIGMS NIH HHS R01 GM102520NIGMS NIH HHS R01 GM110501NIGMS NIH HHS R01 GM117126NIGMS NIH HHS R56 GM055302NIGMS NIH HHS S06 GM008003NIGMS NIH HHS T32 GM008280Wellcome Trust
Abstract
X-ray crystallography at X-ray free-electron laser sources is a powerful method for studying macromolecules at biologically relevant temperatures. Moreover, when combined with complementary techniques like X-ray emission spectroscopy, both global structures and chemical properties of metalloenzymes can be obtained concurrently, providing insights into the interplay between the protein structure and dynamics and the chemistry at an active site. The implementation of such a multimodal approach can be compromised by conflicting requirements to optimize each individual method. In particular, the method used for sample delivery greatly affects the data quality. We present here a robust way of delivering controlled sample amounts on demand using acoustic droplet ejection coupled with a conveyor belt drive that is optimized for crystallography and spectroscopy measurements of photochemical and chemical reactions over a wide range of time scales. Studies with photosystem II, the phytochrome photoreceptor, and ribonucleotide reductase R2 illustrate the power and versatility of this method.
Indexed as
LasersAcousticsCrystallography, X-RayPhotosystem II Protein ComplexPhytochromeRibonucleotide ReductasesSpectrometry, X-Ray EmissionPhotosystem II Protein ComplexPhytochromeRibonucleotide Reductases
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