ArticleScientific reports2017
Medaka insulin-like growth factor-2 supports self-renewal of the embryonic stem cell line and blastomeres in vitro.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 32 citations in OpenAlex.
- Towards Cost-Effective and Sustainable Media Formulations for Terrestrial and Aquatic Cellular Agriculture.Foods (Basel, Switzerland) · 2026Review
- Establishment and Characterization of a Long-Term Ovarian Cell Line (SBO) from Asian Seabass (International journal of molecular sciences · 2026Article
- Insulin-like Growth Factor 2 Regulates Skeletal Muscle Development in Spotted Sea Bass (Lateolabrax maculatus).Marine biotechnology (New York, N.Y.) · 2026Article
- Integrated lncRNA and mRNA Transcriptome Analyses of IGF1 and IGF2 Stimulated Ovaries Reveal Genes and Pathways Potentially Associated with Ovarian Development and Oocyte Maturation in Golden Pompano (Animals : an open access journal from MDPI · 2025Article
- Diversity and dynamics of fish ovaries: Insights into reproductive strategies, hormonal regulation, and ovarian development.Histology and histopathology · 2025Review
- Rat embryonic stem cell-basedFrontiers in toxicology · 2025Article
- Cell-cultivated aquatic food products: emerging production systems for seafood.Journal of biological engineering · 2024Review
- Continuous fish muscle cell line with capacity for myogenic and adipogenic-like phenotypes.Scientific reports · 2023Article
- Molecular Cloning, Screening of Single Nucleotide Polymorphisms, and Analysis of Growth-Associated Traits ofAnimals : an open access journal from MDPI · 2023Article
- Differentiation and Maturation of Muscle and Fat Cells in Cultivated Seafood: Lessons from Developmental Biology.Marine biotechnology (New York, N.Y.) · 2023Review
- Differential Expression of Duplicate Insulin-like Growth Factor-1 Receptors (Life (Basel, Switzerland) · 2022Article
- Insulin-like growth factor signalling and its significance as a biomarker in fish and shellfish research.Fish physiology and biochemistry · 2021Review
- Roles of insulin-like growth factor II in regulating female reproductive physiology.Science China. Life sciences · 2020Review
- An epigenetic screening determines BET proteins as targets to suppress self-renewal and tumorigenicity in canine mammary cancer cells.Scientific reports · 2019Article
- Molecular characterization and expression profiles of insulin-like growth factors in yellowtail kingfish (Seriola lalandi) during embryonic development.Fish physiology and biochemistry · 2019Article
- Data for evolutive analysis of insulin related peptides in bilaterian species.Data in brief · 2019Article
- The insulin-like growth factor 2 gene and locus in nonmammalian vertebrates: Organizational simplicity with duplication but limited divergence in fish.The Journal of biological chemistry · 2018Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Insulin-like growth factors (IGFs) regulate diverse processes including energy metabolism, cell proliferation and embryonic development. They activate the IGF signaling pathway via binding to cell surface receptors. Here we report an essential role of IGF2 in maintaining the pluripotency of embryonic stem (ES) cell from medaka (Oryzias latipes). The medaka igf2 gene was cloned for prokaryotically expression of IGF2 ligand and green fluorescent protein-tagged IGF2 namely IGF2:GFP. With flow cytometry analysis, we demonstrated that the IGF2:GFP can bind to the cultured ES cells from medaka and zebrafish respectively. We also verified that IGF2 is able to activate the phosphorylation of Erk1/2 and Akt, and sustain the viability and pluripotency of medaka ES cells in culture. Furthermore, we characterized the binding of IGF2:GFP to freshly isolated blastomeres by fluorescence microscopy and electron microscopy. Most importantly, we revealed the important role of IGF2 in supporting the derivation of blastomeres in short-term culture. Therefore, Medaka IGF2 is essential for the self-renewal of cultured ES cells and blastomeres from fish embryos. This finding underscores a conserved role of the IGF signaling pathway in stem cells from fish to mammals.
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