Evidence map›Paper›PMID 28249997›Full record

ArticleJournal of clinical microbiology2017

Direct Detection and Identification of Bacterial Pathogens from Urine with Optimized Specimen Processing and Enhanced Testing Algorithm.

Bin Huang, Lei Zhang, Weizheng Zhang, Kang Liao, Shihong Zhang, Zhiquan Zhang, Xingyan Ma, Jialong Chen, Xiuhong Zhang, Pinghua Qu and 3 more

Open access · bronzeAbstract read
In one paragraph

Article in Journal of clinical microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed, 1 pooled it
3.4field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 1 synthesis or guideline pooled it, 33 citations in OpenAlex.

  1. Pooled it
  2. Guidelines for Antibiotics Prescription in Critically Ill Patients.Indian journal of critical care medicine : peer-reviewed, official publication of Indian Society of Critical Care Medicine · 2024
    Article
  3. Article
  4. Article
  5. Review
  6. Rapid identification of uropathogens by combining Alfred 60 system with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry technology.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020
    Article
  7. Article
  8. Article
  9. Review
  10. Article
  11. Article
  12. Article
  13. Article
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  15. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 6 institutions in 2 countries.

Bin HuangDepartment of Laboratory Medicine, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
Lei ZhangKingMed Diagnostics, Guangzhou, China.
Weizheng ZhangDepartment of Laboratory Medicine, Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
Kang LiaoDepartment of Laboratory Medicine, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
Shihong ZhangDepartment of Laboratory Medicine, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
Zhiquan ZhangDepartment of Laboratory Medicine, Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Xingyan MaDepartment of Laboratory Medicine, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
Jialong ChenDepartment of Laboratory Medicine, People's Hospital of Huangpu, Zhongshan, China.
Xiuhong ZhangDepartment of Laboratory Medicine, People's Hospital of Huidong, Huizhou, China.
Pinghua QuDepartment of Laboratory Medicine, Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
Shangwei WuKingMed Diagnostics, Guangzhou, China.
Cha ChenDepartment of Laboratory Medicine, Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China chencha906@163.com tangy@mskcc.org.
Yi-Wei TangDepartment of Laboratory Medicine, Memorial Sloan Kettering Cancer Center, New York, New York, USA chencha906@163.com tangy@mskcc.org.ORCID 0000-0003-4888-6771
Guangzhou University of Chinese Medicine · CNSun Yat-sen University · CNKingmed Diagnostics · CNMemorial Sloan Kettering Cancer Center · USHuizhou Central People's Hospital · CNSecond Affiliated Hospital of Guangzhou Medical University · CN

Funding

X-RAY CRYSTALLOGRAPHYP30CA008748 · NCI · SLOAN-KETTERING INSTITUTE FOR CANCER RES · PI Nicholas D Socci · 1985 to 2026
$347.4M
NCI NIH HHS P30 CA008748
6 · The paper itself

Abstract

Rapid and accurate detection and identification of microbial pathogens causing urinary tract infections allow prompt and specific treatment. We optimized specimen processing to maximize the limit of detection (LOD) by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and evaluated the capacity of combination of MALDI-TOF MS and urine analysis (UA) for direct detection and identification of bacterial pathogens from urine samples. The optimal volumes of processed urine, formic acid/acetonitrile, and supernatant spotted onto the target plate were 15 ml, 3 μl, and 3 μl, respectively, yielding a LOD of 1.0 × 10

Indexed as

AlgorithmsBacteriaBacteriological TechniquesEnterococcus faecalisEscherichia coliFemaleHumansKlebsiella pneumoniaeLimit of DetectionMaleRNA, Ribosomal, 16SSensitivity and SpecificitySpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationUrinalysisUrinary Tract InfectionsUrineRNA, Ribosomal, 16SidentificationMALDI-TOF MSpathogenurineurine analysis

Identifiers

PMID28249997
PMCPMC5405266
OpenAlexW2592951860

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.