ArticleScientific data2017
Genome-wide siRNA screen of genes regulating the LPS-induced TNF-α response in human macrophages.
Article in Scientific data, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed, 15 citations in OpenAlex.
- Identification of potential crucial genes and biomarkers from neutrophils in sepsis using bioinformatics analysis.Medicine · 2025Article
- Multi-Omics Strategies Uncover Host-Pathogen Interactions.ACS infectious diseases · 2019Review
- Using a Systems Biology Approach To Study Host-Pathogen Interactions.Microbiology spectrum · 2019Review
- IFIT1 Exerts Opposing Regulatory Effects on the Inflammatory and Interferon Gene Programs in LPS-Activated Human Macrophages.Cell reports · 2018Article
- Knocking down the obstacles to functional genomics data sharing.Scientific data · 2017Article
- Multidimensional pooled shRNA screens in human THP-1 cells identify candidate modulators of macrophage polarization.PloS one · 2017Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The mammalian innate immune system senses many bacterial stimuli through the toll-like receptor (TLR) family. Activation of the TLR4 receptor by bacterial lipopolysaccharide (LPS) is the most widely studied TLR pathway due to its central role in host responses to gram-negative bacterial infection and its contribution to endotoxemia and sepsis. Here we describe a genome-wide siRNA screen to identify genes regulating the human macrophage TNF-α response to LPS. We include a secondary validation screen conducted with six independent siRNAs per gene to facilitate removal of off-target screen hits. We also provide microarray data from the same LPS-treated macrophage cells to facilitate downstream data analysis. Tertiary screening with multiple TLR ligands and a microbial extract demonstrate that novel screen hits have broad effects on the innate inflammatory response to microbial stimuli. These data provide a resource for analyzing gene function in the predominant pathway driving inflammatory cytokine expression in human macrophages.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.