ArticleScientific data2017
Development of improved vaccine cell lines against rotavirus.
Article in Scientific data, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
- Optimization of YF17D-Vectored Zika Vaccine Production by Employing Small-Molecule Viral Sensitizers to Enhance Yields.Vaccines · 2025Article
- Seed Train Optimization in Microcarrier-Based Cell Culture Post In Situ Cell Detachment through Scale-Down Hybrid Modeling.Bioengineering (Basel, Switzerland) · 2024Article
- Comparison of IgA Antibody Titer Induced by Human-Bovine Rotavirus Candidate Vaccine with Bovine Rotavirus and Rotarix.Archives of Razi Institute · 2023Article
- Repurposing Probenecid to Inhibit SARS-CoV-2, Influenza Virus, and Respiratory Syncytial Virus (RSV) Replication.Viruses · 2022Review
- MicroRNAs affect GPCR and Ion channel genes needed for influenza replication.The Journal of general virology · 2021Article
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- G-Protein-Coupled Receptor and Ion Channel Genes Used by Influenza Virus for Replication.Journal of virology · 2021Article
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- Vero cell upstream bioprocess development for the production of viral vectors and vaccines.Biotechnology advances · 2020Review
- Cell Cultures for Virology: Usability, Advantages, and Prospects.International journal of molecular sciences · 2020Review
- CRISPR-Csy4-Mediated Editing of Rotavirus Double-Stranded RNA Genome.Cell reports · 2020Article
- A pooled genome-wide screening strategy to identify and rank influenza host restriction factors in cell-based vaccine production platforms.Scientific reports · 2020Article
- Article
- Gene-edited vero cells as rotavirus vaccine substrates.Vaccine: X · 2019Article
- Enhancing viral vaccine production using engineered knockout vero cell lines - A second look.Vaccine · 2018Article
- Knocking down the obstacles to functional genomics data sharing.Scientific data · 2017Article
- A universal mammalian vaccine cell line substrate.PloS one · 2017Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rotavirus is a major cause of severe gastroenteritis among very young children. In developing countries, rotavirus is the major cause of mortality in children under five years old, causing up to 20% of all childhood deaths in countries with high diarrheal disease burden, with more than 90% of these deaths occurring in Africa and Asia. Rotavirus vaccination mimics the first infection without causing illness, thus inducing strong and broad heterotypic immunity against prospective rotavirus infections. Two live vaccines are available, Rotarix and RotaTeq, but vaccination efforts are hampered by high production costs. Here, we present a dataset containing a genome-wide RNA interference (RNAi) screen that identified silencing events that enhanced rotavirus replication. Evaluated against several rotavirus vaccine strains, hits were validated in a Vero vaccine cell line as well as CRISPR/Cas9 generated cells permanently and stably lacking the genes that affect RV replication. Knockout cells were dramatically more permissive to RV replication and permitted an increase in rotavirus replication. These data show a means to improve manufacturing of rotavirus vaccine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.