ArticleNature microbiology2017
A global analysis of transcription reveals two modes of Spt4/5 recruitment to archaeal RNA polymerase.
Article in Nature microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 43 citations in OpenAlex.
- Elucidating the interplay between intracellular manganese-iron-ratio and radiotolerance across all domains of life.FEMS microbes · 2026Article
- Chromatin and gene regulation in archaea.Molecular microbiology · 2025Review
- Structural basis of archaeal RNA polymerase transcription elongation and Spt4/5 recruitment.Nucleic acids research · 2024Article
- DNA-bridging by an archaeal histone variant via a unique tetramerisation interface.Communications biology · 2023Article
- Endonucleolytic processing plays a critical role in the maturation of ribosomal RNA inRNA biology · 2023Article
- How to Shut Down Transcription in Archaea during Virus Infection.Microorganisms · 2022Review
- ChIP-Seq Occupancy Mapping of the Archaeal Transcription Machinery.Methods in molecular biology (Clifton, N.J.) · 2022Article
- aCPSF1 cooperates with terminator U-tract to dictate archaeal transcription termination efficacy.eLife · 2021Article
- Promoter-proximal elongation regulates transcription in archaea.Nature communications · 2021Article
- Specific Features of RNA Polymerases I and III: Structure and Assembly.Frontiers in molecular biosciences · 2021Review
- Coupling of Transcription and Translation in Archaea: Cues From the Bacterial World.Frontiers in microbiology · 2021Article
- Archaeal transcription.Transcription · 2020Review
- The conserved ribonuclease aCPSF1 triggers genome-wide transcription termination of Archaea via a 3'-end cleavage mode.Nucleic acids research · 2020Article
- DNA origami-based single-molecule force spectroscopy elucidates RNA Polymerase III pre-initiation complex stability.Nature communications · 2020Article
- Recent Advances in Archaeal Translation Initiation.Frontiers in microbiology · 2020Review
- Article
- The archaeal RNA chaperone TRAM0076 shapes the transcriptome and optimizes the growth of Methanococcus maripaludis.PLoS genetics · 2019Article
- The Primary Antisense Transcriptome ofGenes · 2019Article
- Next Generation DNA-Seq and Differential RNA-Seq Allow Re-annotation of theFrontiers in microbiology · 2019Article
- A Genetic System forFrontiers in microbiology · 2019Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The archaeal transcription apparatus is closely related to the eukaryotic RNA polymerase (RNAP) II system, while archaeal genomes are more similar to bacteria with densely packed genes organized in operons. This makes understanding transcription in archaea vital, both in terms of molecular mechanisms and evolution. Very little is known about how archaeal cells orchestrate transcription on a systems level. We have characterized the genome-wide occupancy of the Methanocaldococcus jannaschii transcription machinery and its transcriptome. Our data reveal how the TATA and BRE promoter elements facilitate recruitment of the essential initiation factors TATA-binding protein and transcription factor B, respectively, which in turn are responsible for the loading of RNAP into the transcription units. The occupancies of RNAP and Spt4/5 strongly correlate with each other and with RNA levels. Our results show that Spt4/5 is a general elongation factor in archaea as its presence on all genes matches RNAP. Spt4/5 is recruited proximal to the transcription start site on the majority of transcription units, while on a subset of genes, including rRNA and CRISPR loci, Spt4/5 is recruited to the transcription elongation complex during early elongation within 500 base pairs of the transcription start site and akin to its bacterial homologue NusG.
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