ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2017
Group B streptococcal PCR testing in comparison to culture for diagnosis of late onset bacteraemia and meningitis in infants aged 7-90 days: a multi-centre diagnostic accuracy study.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 26 citations in OpenAlex.
- Molecular assays for the diagnosis of sepsis in neonates: a diagnostic test accuracy review.The Cochrane database of systematic reviews · 2025Pooled it
- Enhanced identification of Group B streptococcus in infants with suspected meningitis in Ethiopia.PloS one · 2020Trial
- Group BLancet regional health. Americas · 2025Article
- Molecular diagnostics in cerebrospinal fluid for the diagnosis of central nervous system infections.Clinical microbiology reviews · 2024Review
- Burden of infant group BLancet regional health. Americas · 2024Article
- Lumbar Puncture and Meningitis in Infants with Proven Early- or Late-Onset Sepsis: An Italian Prospective Multicenter Observational Study.Microorganisms · 2023Article
- Predicting treatment response to vancomycin using bacterial DNA load as a pharmacodynamic marker in premature and very low birth weight neonates: A population PKPD study.Frontiers in pharmacology · 2023Article
- Mania: An atypical presentation of probableIDCases · 2023Article
- Group B Streptococcal Neonatal Meningitis.Clinical microbiology reviews · 2022Review
- Multiplex Droplet Digital Polymerase Chain Reaction Assay for Rapid Molecular Detection of Pathogens in Patients With Sepsis: Protocol for an Assay Development Study.JMIR research protocols · 2021Article
- Pediatric sepsis cases diagnosed with group B streptococcal meningitis using next-generation sequencing: a report of two cases.BMC infectious diseases · 2021Article
- Case Report: Prolonged CSF PCR Positivity in a Neonate With GBS Meningitis.Frontiers in pediatrics · 2021Article
- Towards accurate exclusion of neonatal bacterial meningitis: a feasibility study of a novel 16S rDNA PCR assay.BMC infectious diseases · 2020Article
- Clinical characteristics and molecular epidemiology of children with meningitis in Tehran, Iran: a prospective study.New microbes and new infections · 2019Article
- Disease burden of neonatal invasive Group B Streptococcus infection in the Netherlands.PloS one · 2019Article
- Neonatal Meningitis: Overcoming Challenges in Diagnosis, Prognosis, and Treatment with Omics.Frontiers in pediatrics · 2017Review
Corrections and comments
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Authors and funding
12 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to compare an in-house real-time PCR assay, with bacterial culture as the reference, for the diagnosis of late onset group B Streptococcal (GBS) disease. This was a retrospective review. All children aged 7-90 days presenting to four paediatric centres that had a blood or CSF sample tested by GBS PCR were included. Of 7,686 blood and 2,495 cerebrospinal fluid (CSF) samples from patients of all ages received for PCR testing, 893 and 859 samples were eligible for the study, respectively. When compared to culture, the sensitivity of blood PCR was 65% (13/20) in comparison to the CSF PCR test which was 100% (5/5). Ten of 23 PCR-positive blood samples and 17 of 22 PCR-positive CSF samples were culture negative. The median threshold Ct values for culture-positive/PCR-positive CSF samples was lower than that of culture-negative/PCR-positive CSF samples (p = 0.08). Clinical details of 17 available cases that were culture negative/PCR positive were reviewed; seven were deemed to be definite cases, eight were probable and two were possible. The results showed that detection of GBS by PCR is useful for CSF samples from infants aged 7-90 days with suspected meningitis; however, analysis of blood samples by PCR is of limited value as a routine screening test for late onset GBS sepsis and should not replace bacterial culture.
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