ArticleScientific reports2017
Quantitative Limits on Small Molecule Transport via the Electropermeome - Measuring and Modeling Single Nanosecond Perturbations.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Simulation of intracellular delivery through permeabilized multivesicular vesicles.European biophysics journal : EBJ · 2026Article
- Optimizing electroporation via pulse modulation: a molecular dynamics study.European biophysics journal : EBJ · 2025Article
- Interleaflet Translocation of Second-Harmonic-Generation-Active Dye Molecules in Phospholipid Bilayers with Transmembrane Pores.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Modulating CaBiophysical journal · 2024Article
- Real-time imaging of individual electropores proves their longevity in cells.Biochemical and biophysical research communications · 2024Article
- Assessing membrane material properties from the response of giant unilamellar vesicles to electric fields.Advances in physics: X · 2023Article
- Dye Transport through Bilayers Agrees with Lipid Electropore Molecular Dynamics.Biophysical journal · 2020Article
- Probing Nanoelectroporation and Resealing of the Cell Membrane by the Entry of CaInternational journal of molecular sciences · 2020Article
- Article
- Cancellation of nerve excitation by the reversal of nanosecond stimulus polarity and its relevance to the gating time of sodium channels.Cellular and molecular life sciences : CMLS · 2019Article
- Application of Pulsed Electric Fields to Cancer Therapy.Bioelectricity · 2019Review
- The second phase of bipolar, nanosecond-range electric pulses determines the electroporation efficiency.Bioelectrochemistry (Amsterdam, Netherlands) · 2018Article
- Excitation and injury of adult ventricular cardiomyocytes by nano- to millisecond electric shocks.Scientific reports · 2018Article
- Different Membrane Pathways Mediate CaBioMed research international · 2018Article
- Transport of charged small molecules after electropermeabilization - drift and diffusion.BMC biophysics · 2018Article
- Neuronal excitation and permeabilization by 200-ns pulsed electric field: An optical membrane potential study with FluoVolt dye.Biochimica et biophysica acta. Biomembranes · 2017Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The detailed molecular mechanisms underlying the permeabilization of cell membranes by pulsed electric fields (electroporation) remain obscure despite decades of investigative effort. To advance beyond descriptive schematics to the development of robust, predictive models, empirical parameters in existing models must be replaced with physics- and biology-based terms anchored in experimental observations. We report here absolute values for the uptake of YO-PRO-1, a small-molecule fluorescent indicator of membrane integrity, into cells after a single electric pulse lasting only 6 ns. We correlate these measured values, based on fluorescence microphotometry of hundreds of individual cells, with a diffusion-based geometric analysis of pore-mediated transport and with molecular simulations of transport across electropores in a phospholipid bilayer. The results challenge the "drift and diffusion through a pore" model that dominates conventional explanatory schemes for the electroporative transfer of small molecules into cells and point to the necessity for a more complex model.
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