Evidence map›Paper›PMID 28245594›Full record

ArticleToxins2017

Development of Colloidal Gold-Based Lateral Flow Immunoassay for Rapid Qualitative and SemiQuantitative Analysis of Ustiloxins A and B in Rice Samples.

Xiaoxiang Fu, Rushan Xie, Jian Wang, Xiaojiao Chen, Xiaohan Wang, Weibo Sun, Jiajia Meng, Daowan Lai, Ligang Zhou, Baomin Wang

Open access · goldAbstract read
In one paragraph

Article in Toxins, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
8.1field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 36 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Quantitative Loop-Mediated Isothermal Amplification Detection ofInternational journal of molecular sciences · 2023
    Article
  5. Review
  6. Article
  7. The Conserved Effector UvHrip1 interacts with OsHGW, and Infection ofInternational journal of molecular sciences · 2020
    Article
  8. Frontiers in microbiology · 2020
    Article
  9. Article
  10. A Novel Effector GeneFrontiers in microbiology · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Xiaoxiang FuDepartment of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing 100193, China. xioxiaofu@cau.edu.cn.
Rushan XieDepartment of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing 100193, China. erinxrs@163.com.
Jian WangDepartment of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing 100193, China. wangjiancau@163.com.
Xiaojiao ChenDepartment of Crop Physiology and Cultivation, College of Agronomy and Biotechnology, Beijing 100193, China. chenxj@126.com.
Xiaohan WangDepartment of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing 100193, China. wangxiaohan99@126.com.
Weibo SunDepartment of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing 100193, China. sunweibo.1001@163.com.
Jiajia MengDepartment of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing 100193, China. mengjiajiax@163.com.
Daowan LaiDepartment of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing 100193, China. dwlai@cau.edu.cn.
Ligang ZhouDepartment of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing 100193, China. lgzhou@cau.edu.cn.
Baomin WangDepartment of Crop Physiology and Cultivation, College of Agronomy and Biotechnology, Beijing 100193, China. wbaomin@263.net.
China Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Rice false smut is a worldwide devastating rice disease infected by the fungal pathogen Villosiclava virens. Ustiloxin A (UA) and ustiloxin B (UB), cyclopeptide mycotoxins, were the major ustiloxins isolated from the rice false smut balls (FSBs) that formed in the pathogen-infected rice spikelets. Based on the specific monoclonal antibodies (mAbs) 2D3G5 and 1B5A10, respectively, against UA and UB, the lateral flow immunoassays (LFIAs) were developed, and the indicator ranges for UA and UB both were 50-100 ng/mL. The cross-reactivities of UB for UA LFIA, and UA for UB LFIA were 5% and 20%, respectively, which were consistent with the icELISA results reported previously. Even at 50,000 ng/mL, none of other commonly existent metabolites in rice samples caused noticeable inhibition. The LFIAs were used for determination of UA and UB contents in rice FSBs and rice grains, and the results were agreeable with those by HPLC and icELISA. There was no change in the sensitivity of either dipstick stored at 4 °C) after at least three months. The developed LFIA has specificity and sensitivity for detecting UA and UB as well as simplicity to use. It will be a potential point-of-care device for rapid evaluation of the rice samples contaminated by UA and UB.

Indexed as

Gold ColloidAntibodies, MonoclonalImmunoassayMycotoxinsOryzaPeptides, CyclicAntibodies, MonoclonalGold ColloidMycotoxinsPeptides, Cyclicustiloxin Austiloxin Blateral flow immunoassaymycotoxinrice false smut ballsrice false smut diseaserice grainsustiloxins A and BVillosiclava virens

Identifiers

PMID28245594
PMCPMC5371834
OpenAlexW2589181972

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.