ArticleJournal of proteome research2017
Optimization and Application of Direct Infusion Nanoelectrospray HRMS Method for Large-Scale Urinary Metabolic Phenotyping in Molecular Epidemiology.
Article in Journal of proteome research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 51 citations in OpenAlex.
- Single-Injection Multi-Omics Analysis by Direct Infusion Mass Spectrometry.Angewandte Chemie (International ed. in English) · 2026Article
- Standard Addition as a Method for Quantitative Mass Spectrometry Imaging.Analytical chemistry · 2025Article
- Review
- A Strategy for Uncovering the Serum Metabolome by Direct-Infusion High-Resolution Mass Spectrometry.Metabolites · 2023Article
- Large-Scale Interlaboratory DI-FT-ICR MS Comparability Study Employing Various Systems.Journal of the American Society for Mass Spectrometry · 2022Article
- Sunlight can convert atmospheric aerosols into a glassy solid state and modify their environmental impacts.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Rapid tryptic peptide mapping of human serum albumin using DI-MS/MSRSC advances · 2022Article
- Defining Acute Coronary Syndrome through Metabolomics.Metabolites · 2021Review
- Metabolomic Profile in Venous Thromboembolism (VTE).Metabolites · 2021Review
- Acoustic Mist Ionization Mass Spectrometry for Ultrahigh-Throughput Metabolomics Screening.Analytical chemistry · 2021Article
- Omics-Driven Biotechnology for Industrial Applications.Frontiers in bioengineering and biotechnology · 2021Review
- Quantitative shotgun proteome analysis by direct infusion.Nature methods · 2020Article
- Deriving Lipid Classification Based on Molecular Formulas.Metabolites · 2020Article
- High-Throughput Nanoelectrospray Ionization-Mass Spectrometry Analysis of Microfluidic Droplet Samples.Analytical chemistry · 2019Article
- Ultra-Performance Liquid Chromatography-High-Resolution Mass Spectrometry and Direct Infusion-High-Resolution Mass Spectrometry for Combined Exploratory and Targeted Metabolic Profiling of Human Urine.Journal of proteome research · 2018Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Large-scale metabolic profiling requires the development of novel economical high-throughput analytical methods to facilitate characterization of systemic metabolic variation in population phenotypes. We report a fit-for-purpose direct infusion nanoelectrospray high-resolution mass spectrometry (DI-nESI-HRMS) method with time-of-flight detection for rapid targeted parallel analysis of over 40 urinary metabolites. The newly developed 2 min infusion method requires <10 μL of urine sample and generates high-resolution MS profiles in both positive and negative polarities, enabling further data mining and relative quantification of hundreds of metabolites. Here we present optimization of the DI-nESI-HRMS method in a detailed step-by-step guide and provide a workflow with rigorous quality assessment for large-scale studies. We demonstrate for the first time the application of the method for urinary metabolic profiling in human epidemiological investigations. Implementation of the presented DI-nESI-HRMS method enabled cost-efficient analysis of >10 000 24 h urine samples from the INTERMAP study in 12 weeks and >2200 spot urine samples from the ARIC study in <3 weeks with the required sensitivity and accuracy. We illustrate the application of the technique by characterizing the differences in metabolic phenotypes of the USA and Japanese population from the INTERMAP study.
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