ArticleThe Journal of cell biology1987
Cyclic AMP functions as a primary sexual signal in gametes of Chlamydomonas reinhardtii.
Article in The Journal of cell biology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
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Who cites it
81 citing papers in PubMed, 217 citations in OpenAlex.
- Aspects of plant intelligence (ii).Plant signaling & behavior · 2026Review
- Stereotypical interciliary contacts in abioRxiv : the preprint server for biology · 2026Article
- Extensive remodeling during Chlamydomonas reinhardtii zygote maturation leads to highly resistant zygospores.The Plant journal : for cell and molecular biology · 2025Article
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- Light-independent regulation of algal photoprotection by CONature communications · 2023Article
- Initial ciliary assembly inMolecular biology of the cell · 2023Article
- A gap-free genome assembly of Chlamydomonas reinhardtii and detection of translocations induced by CRISPR-mediated mutagenesis.Plant communications · 2023Article
- Uncovering an ancestral green ménage à trois: Contributions of Chlamydomonas to the discovery of a broadly conserved triad of plant fertilization proteins.Current opinion in plant biology · 2022Review
- A cytoplasmic protein kinase couples engagement of Chlamydomonas ciliary receptors to cAMP-dependent cellular responses.Journal of cell science · 2022Article
- Characterization of the cAMP phosphodiesterase domain in plant adenylyl cyclase/cAMP phosphodiesterase CAPE from the liverwort Marchantia polymorpha.Journal of plant research · 2022Article
- A dynein-associated photoreceptor protein prevents ciliary acclimation to blue light.Science advances · 2021Article
- HAP2-Mediated Gamete Fusion: Lessons From the World of Unicellular Eukaryotes.Frontiers in cell and developmental biology · 2021Review
- Novel Modular Rhodopsins from Green Algae Hold Great Potential for Cellular Optogenetic Modulation Across the Biological Model Systems.Life (Basel, Switzerland) · 2020Article
- TransgenicFrontiers in pharmacology · 2020Article
- Transient Internalization and Microtubule-Dependent Trafficking of a Ciliary Signaling Receptor from the Plasma Membrane to the Cilium.Current biology : CB · 2019Article
- A Series of Fortunate Events: Introducing Chlamydomonas as a Reference Organism.The Plant cell · 2019Review
- Composition, structure and function of the eukaryotic flagellum distal tip.Essays in biochemistry · 2018Review
- In vivo analysis of outer arm dynein transport reveals cargo-specific intraflagellar transport properties.Molecular biology of the cell · 2018Article
- In vivo analyses of radial spoke transport, assembly, repair and maintenance.Cytoskeleton (Hoboken, N.J.) · 2018Article
21 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
When Chlamydomonas reinhardtii gametes of opposite mating type are mixed together, they adhere by a flagella-mediated agglutination that triggers three rapid mating responses: flagellar tip activation, cell wall loss, and mating structure activation accompanied by actin polymerization. Here we show that a transient 10-fold elevation of intracellular cAMP levels is also triggered by sexual agglutination. We further show that gametes of a single mating type can be induced to undergo all three mating responses when presented with exogenous dibutyryl-cAMP (db-cAMP). These events are also induced by cyclic nucleotide phosphodiesterase inhibitors, which elevate endogenous cAMP levels and act synergistically with db-cAMP. Non-agglutinating mutants of opposite mating type will fuse efficiently in the presence of db-cAMP. No activation of mating events is induced by calcium plus ionophores, 8-bromo-cGMP, dibutyryl-cGMP, nigericin at alkaline pH, phorbol esters, or forskolin. H-8, an inhibitor of cyclic nucleotide-dependent protein kinase, inhibits mating events in agglutinating cells and antagonizes the effects of cAMP on non-agglutinating cells. Adenylate cyclase activity was detected in both the gamete cell body and flagella, with the highest specific activity displayed in flagellar membrane fractions. The flagellar membrane adenylate cyclase is preferentially stimulated by Mn++, unresponsive to NaF, GTP, GTP gamma S, AlF4-, and forskolin, and is inhibited by trifluoperazine. Cyclic nucleotide phosphodiesterase activity is also present in flagella. Our observations indicate that cAMP is a sufficient initial signal for all of the known mating reaction events in C. reinhardtii, and suggest that the flagellar cyclase and/or phosphodiesterase may be important loci of control for the agglutination-stimulated production of this signal.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.