ArticleWorld journal of microbiology & biotechnology2017
Construction of pyruvate producing strain with intact pyruvate dehydrogenase and genome-wide transcription analysis.
Article in World journal of microbiology & biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 27 citations in OpenAlex.
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- An Analysis of the Mechanism About COInternational journal of molecular sciences · 2024Article
- FastKnock: an efficient next-generation approach to identify all knockout strategies for strain optimization.Microbial cell factories · 2024Article
- Enhancement of acid tolerance of Escherichia coli by introduction of molecule chaperone CbpA from extremophile.World journal of microbiology & biotechnology · 2023Article
- Erwinia amylovora Auxotrophic Mutant Exometabolomics and Virulence on Apples.Applied and environmental microbiology · 2019Article
- Improved xylose tolerance and 2,3-butanediol production ofBiotechnology for biofuels · 2018Article
- Holistic bioengineering: rewiring central metabolism for enhanced bioproduction.The Biochemical journal · 2017Review
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Authors and funding
2 authors at 2 institutions in 1 country.
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Abstract
To obtain strain YP211 with a high tendency for accumulating pyruvate, central metabolic pathways were modified in Escherichia coli MG1655. Specifically, seven genes (ldhA, pflB, pta-ackA, poxB, ppc, frdBC) were knocked out sequentially and full pyruvate dehydrogenase was retained. In batch fermentation with M9 medium, pyruvate yield and production rate reached 0.63 g/g glucose and 1.89 g/(1 h), respectively. Meanwhile, the production of acetate, succinate, and other carboxylates was effectively controlled. To understand the physiological observations, we further completed genome-wide transcription analysis of wild-type and YP211. As the acetic acid pathways were blocked, the pathways of convertion of pyruvate to phosphoenol pyruvate and acetyl CoA were enhanced. The transcription of pck, as an alternative gene for ppc, was increased by 2.6 times. So even if gene ppc was inactivated, the tricarboxylic acid pathway was still enhanced in YP211. In order to balance intracellular NADH/NAD
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